ArticleProceedings of the National Academy of Sciences of the United States of America2014
Chloride channels in stellate cells are essential for uniquely high secretion rates in neuropeptide-stimulated Drosophila diuresis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 84 citations in OpenAlex.
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- Compromised junctional integrity phenocopies age-dependent renal dysfunction in Drosophila Snakeskin mutants.Journal of cell science · 2023Article
- Drosophila melanogaster: a simple genetic model of kidney structure, function and disease.Nature reviews. Nephrology · 2022Review
- Transcriptional and functional motifs defining renal function revealed by single-nucleus RNA sequencing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Review
- Tumour-host interactions through the lens of Drosophila.Nature reviews. Cancer · 2021Review
- Updates on ion and water transport by the Malpighian tubule.Current opinion in insect science · 2021Review
- Transporters and tubule crystals in the insect Malpighian tubule.Current opinion in insect science · 2021Review
- Molecular basis for epithelial morphogenesis and ion transport in the Malpighian tubule.Current opinion in insect science · 2021Review
- A unique Malpighian tubule architecture inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Leucokinin and Associated Neuropeptides Regulate Multiple Aspects of Physiology and Behavior inInternational journal of molecular sciences · 2021Review
- Leucokinins: Multifunctional Neuropeptides and Hormones in Insects and Other Invertebrates.International journal of molecular sciences · 2021Review
- The septate junction protein Mesh is required for epithelial morphogenesis, ion transport, and paracellular permeability in theAmerican journal of physiology. Cell physiology · 2020Article
- Physiology, Development, and Disease Modeling in theGenetics · 2020Review
- Specialized stellate cells offer a privileged route for rapid water flux inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- SIK3 suppresses neuronal hyperexcitability by regulating the glial capacity to buffer KThe Journal of cell biology · 2019Article
- Article
- Targeted renal knockdown of NaAmerican journal of physiology. Renal physiology · 2019Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Epithelia frequently segregate transport processes to specific cell types, presumably for improved efficiency and control. The molecular players underlying this functional specialization are of particular interest. In Drosophila, the renal (Malpighian) tubule displays the highest per-cell transport rates known and has two main secretory cell types, principal and stellate. Electrogenic cation transport is known to reside in the principal cells, whereas stellate cells control the anion conductance, but by an as-yet-undefined route. Here, we resolve this issue by showing that a plasma membrane chloride channel, encoded by ClC-a, is exclusively expressed in the stellate cell and is required for Drosophila kinin-mediated induction of diuresis and chloride shunt conductance, evidenced by chloride ion movement through the stellate cells, leading to depolarization of the transepithelial potential. By contrast, ClC-a knockdown had no impact on resting secretion levels. Knockdown of a second CLC gene showing highly abundant expression in adult Malpighian tubules, ClC-c, did not impact depolarization of transepithelial potential after kinin stimulation. Therefore, the diuretic action of kinin in Drosophila can be explained by an increase in ClC-a-mediated chloride conductance, over and above a resting fluid transport level that relies on other (ClC-a-independent) mechanisms or routes. This key segregation of cation and anion transport could explain the extraordinary fluid transport rates displayed by some epithelia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.