ArticleGenes & development2014
Drosophila TAP/p32 is a core histone chaperone that cooperates with NAP-1, NLP, and nucleophosmin in sperm chromatin remodeling during fertilization.
Article in Genes & development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
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- Cytoplasmic incompatibility factor proteins fromProceedings. Biological sciences · 2025Article
- Epigenetic inheritance and gene expression regulation in early Drosophila embryos.EMBO reports · 2024Review
- Proliferation and dissemination of killer meiotic drive loci.Current opinion in genetics & development · 2023Review
- Essential and recurrent roles for hairpin RNAs in silencing de novo sex chromosome conflict in Drosophila simulans.PLoS biology · 2023Article
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- CDKN2AIP is critical for spermiogenesis and germ cell development.Cell & bioscience · 2022Article
- Histone Chaperones as Cardinal Players in Development.Frontiers in cell and developmental biology · 2022Review
- The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.PLoS genetics · 2021Article
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- A single-cell atlas of adult Drosophila ovary identifies transcriptional programs and somatic cell lineage regulating oogenesis.PLoS biology · 2020Article
- TheeLife · 2019Article
- Oligomerization of Drosophila Nucleoplasmin-Like Protein is required for its centromere localization.Nucleic acids research · 2018Article
- Cell Receptor and Cofactor Interactions of the Contact Activation System and Factor XI.Frontiers in medicine · 2018Review
- Thioredoxin-dependent disulfide bond reduction is required for protamine eviction from sperm chromatin.Genes & development · 2016Article
- Independent Biological and Biochemical Functions for Individual Structural Domains of Drosophila Linker Histone H1.The Journal of biological chemistry · 2016Article
- Unwind and transcribe: chromatin reprogramming in the early mammalian embryo.Current opinion in genetics & development · 2015Review
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6 authors at 1 institution in 1 country.
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Abstract
Nuclear DNA in the male gamete of sexually reproducing animals is organized as sperm chromatin compacted primarily by sperm-specific protamines. Fertilization leads to sperm chromatin remodeling, during which protamines are expelled and replaced by histones. Despite our increased understanding of the factors that mediate nucleosome assembly in the nascent male pronucleus, the machinery for protamine removal remains largely unknown. Here we identify four Drosophila protamine chaperones that mediate the dissociation of protamine-DNA complexes: NAP-1, NLP, and nucleophosmin are previously characterized histone chaperones, and TAP/p32 has no known function in chromatin metabolism. We show that TAP/p32 is required for the removal of Drosophila protamine B in vitro, whereas NAP-1, NLP, and Nph share roles in the removal of protamine A. Embryos from P32-null females show defective formation of the male pronucleus in vivo. TAP/p32, similar to NAP-1, NLP, and Nph, facilitates nucleosome assembly in vitro and is therefore a histone chaperone. Furthermore, mutants of P32, Nlp, and Nph exhibit synthetic-lethal genetic interactions. In summary, we identified factors mediating protamine removal from DNA and reconstituted in a defined system the process of sperm chromatin remodeling that exchanges protamines for histones to form the nucleosome-based chromatin characteristic of somatic cells.
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