ArticleNucleic acids research2014
AP endonucleases process 5-methylcytosine excision intermediates during active DNA demethylation in Arabidopsis.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- Genotype-dependent DNA methylation patterns are negatively associated with allelic variation rather than heat-induced gene expression in two contrasting potato genotypes.The Plant journal : for cell and molecular biology · 2026Article
- Molecular mechanisms and biological functions of active DNA demethylation in plants.Epigenetics & chromatin · 2025Review
- The transcriptome landscape of Kumrogarh, a unique rice landrace showing the simultaneous presence of3 Biotech · 2025Article
- Molecular Mechanisms Underlying the Establishment, Maintenance, and Removal of DNA Methylation in Plants.Annual review of plant biology · 2025Review
- Review
- Review
- Characterization of DNA methylation reader proteins inGenome research · 2024Article
- The chromatin remodeler ERCC6 and the histone chaperone NAP1 are involved in apurinic/apyrimidinic endonuclease-mediated DNA repair.The Plant cell · 2024Article
- Accumulation of DNA damage alters microRNA gene transcription in Arabidopsis thaliana.BMC plant biology · 2022Article
- Plant DNA Methylation: An Epigenetic Mark in Development, Environmental Interactions, and Evolution.International journal of molecular sciences · 2022Review
- Application of 5-Methylcytosine DNA Glycosylase to the Quantitative Analysis of DNA Methylation.International journal of molecular sciences · 2021Article
- Dynamics of DNA Methylation and Its Functions in Plant Growth and Development.Frontiers in plant science · 2021Review
- Nick-seq for single-nucleotide resolution genomic maps of DNA modifications and damage.Nucleic acids research · 2020Article
- Cytosine Methylation Affects the Mutability of Neighboring Nucleotides in Germline and Soma.Genetics · 2020Article
- Active DNA Demethylation in Plants.International journal of molecular sciences · 2019Review
- ROS1-Dependent DNA Demethylation Is Required for ABA-InduciblePlant physiology · 2019Article
- DNA Base Excision Repair in Plants: An Unfolding Story With Familiar and Novel Characters.Frontiers in plant science · 2019Review
- APURINIC/APYRIMIDINIC ENDONUCLEASE2 and ZINC FINGER DNA 3'-PHOSPHOESTERASE Play Overlapping Roles in the Maintenance of Epigenome and Genome Stability.The Plant cell · 2018Article
- Single-Strand Break End Resection in Genome Integrity: Mechanism and Regulation by APE2.International journal of molecular sciences · 2018Review
- Dynamics and function of DNA methylation in plants.Nature reviews. Molecular cell biology · 2018Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is a primary epigenetic modification regulating gene expression and chromatin structure in many eukaryotes. Plants have a unique DNA demethylation system in that 5-methylcytosine (5mC) is directly removed by DNA demethylases, such as DME/ROS1 family proteins, but little is known about the downstream events. During 5mC excision, DME produces 3'-phosphor-α, β-unsaturated aldehyde and 3'-phosphate by successive β- and δ-eliminations, respectively. The kinetic studies revealed that these 3'-blocking lesions persist for a significant amount of time and at least two different enzyme activities are required to immediately process them. We demonstrate that Arabidopsis AP endonucleases APE1L, APE2 and ARP have distinct functions to process such harmful lesions to allow nucleotide extension. DME expression is toxic to E. coli due to excessive 5mC excision, but expression of APE1L or ARP significantly reduces DME-induced cytotoxicity. Finally, we propose a model of base excision repair and DNA demethylation pathway unique to plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.