ArticleThe Plant cell2014
A root-expressed L-phenylalanine:4-hydroxyphenylpyruvate aminotransferase is required for tropane alkaloid biosynthesis in Atropa belladonna.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 77 citations in OpenAlex.
- A Comprehensive Review on the Biosynthesis of Tropane Alkaloids.Molecules (Basel, Switzerland) · 2026Review
- Genome-Wide Analysis of the Metallocarboxypeptidase Inhibitor Family Reveals ThatInternational journal of molecular sciences · 2024Article
- A chromosome-level genome assembly of anesthetic drug-producing Anisodus acutangulus provides insights into its evolution and the biosynthesis of tropane alkaloids.Plant communications · 2024Article
- Multiple independent losses of the biosynthetic pathway for two tropane alkaloids in the Solanaceae family.Nature communications · 2023Article
- Radiocarbon Flux Measurements Reveal Mechanistic Insight into Heat-Stress Induction of Nicotine Biosynthesis inInternational journal of molecular sciences · 2023Article
- Revealing evolution of tropane alkaloid biosynthesis by analyzing two genomes in the Solanaceae family.Nature communications · 2023Article
- Redirecting tropane alkaloid metabolism reveals pyrrolidine alkaloid diversity in Atropa belladonna.The New phytologist · 2023Article
- Transcriptome Analysis Reveals an Essential Role of Exogenous Brassinolide on the Alkaloid Biosynthesis Pathway in Pinellia Ternata.International journal of molecular sciences · 2022Article
- Fruity, sticky, stinky, spicy, bitter, addictive, and deadly: evolutionary signatures of metabolic complexity in the Solanaceae.Natural product reports · 2022Review
- Metabolomics-guided discovery of cytochrome P450s involved in pseudotropine-dependent biosynthesis of modified tropane alkaloids.Nature communications · 2022Article
- Biotechnological Approaches on Engineering Medicinal Tropane Alkaloid Production in Plants.Frontiers in plant science · 2022Review
- Biosynthesis and synthetic biology of psychoactive natural products.Chemical Society reviews · 2021Review
- Alkaloids of the GenusMolecules (Basel, Switzerland) · 2021Review
- Cell Suspensions ofMolecules (Basel, Switzerland) · 2019Article
- Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids.Nature communications · 2019Article
- Comparative transcriptomic analysis reveal the regulation mechanism underlying MeJA-induced accumulation of alkaloids in Dendrobium officinale.Journal of plant research · 2019Article
- Tropane Alkaloids: Chemistry, Pharmacology, Biosynthesis and Production.Molecules (Basel, Switzerland) · 2019Review
- Metabolic Characterization ofFrontiers in plant science · 2019Article
- Targeted expression of Vitreoscilla hemoglobin improves the production of tropane alkaloids in Hyoscyamus niger hairy roots.Scientific reports · 2018Article
- Tropinone synthesis via an atypical polyketide synthase and P450-mediated cyclization.Nature communications · 2018Article
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
The tropane alkaloids, hyoscyamine and scopolamine, are medicinal compounds that are the active components of several therapeutics. Hyoscyamine and scopolamine are synthesized in the roots of specific genera of the Solanaceae in a multistep pathway that is only partially elucidated. To facilitate greater understanding of tropane alkaloid biosynthesis, a de novo transcriptome assembly was developed for Deadly Nightshade (Atropa belladonna). Littorine is a key intermediate in hyoscyamine and scopolamine biosynthesis that is produced by the condensation of tropine and phenyllactic acid. Phenyllactic acid is derived from phenylalanine via its transamination to phenylpyruvate, and mining of the transcriptome identified a phylogenetically distinct aromatic amino acid aminotransferase (ArAT), designated Ab-ArAT4, that is coexpressed with known tropane alkaloid biosynthesis genes in the roots of A. belladonna. Silencing of Ab-ArAT4 disrupted synthesis of hyoscyamine and scopolamine through reduction of phenyllactic acid levels. Recombinant Ab-ArAT4 preferentially catalyzes the first step in phenyllactic acid synthesis, the transamination of phenylalanine to phenylpyruvate. However, rather than utilizing the typical keto-acid cosubstrates, 2-oxoglutarate, pyruvate, and oxaloacetate, Ab-ArAT4 possesses strong substrate preference and highest activity with the aromatic keto-acid, 4-hydroxyphenylpyruvate. Thus, Ab-ArAT4 operates at the interface between primary and specialized metabolism, contributing to both tropane alkaloid biosynthesis and the direct conversion of phenylalanine to tyrosine.
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