ArticleAnalytical and bioanalytical chemistry2014
Validation of the Endopep-MS method for qualitative detection of active botulinum neurotoxins in human and chicken serum.
Article in Analytical and bioanalytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Botulism Type F, a Rare Type of Botulism that Can Masquerade as a Posterior Fossa Stroke.Journal of medical toxicology : official journal of the American College of Medical Toxicology · 2026Article
- Article
- Review
- New approach for the rational selection of markers to identify botulinum toxins.Archives of toxicology · 2021Article
- Modification and validation of the Endopep-mass spectrometry method for botulinum neurotoxin detection in liver samples with application to samples collected during animal botulism outbreaks.Analytical and bioanalytical chemistry · 2021Article
- Toxemia in Human Naturally Acquired Botulism.Toxins · 2020Review
- Alternative Methods for Testing Botulinum Toxin: Current Status and Future Perspectives.Biomolecules & therapeutics · 2020Review
- Article
- Botulinum Neurotoxin Detection Methods for Public Health Response and Surveillance.Frontiers in bioengineering and biotechnology · 2018Review
- Development of a multiplex Endopep-MS assay for simultaneous detection of botulinum toxins A, B and E.Scientific reports · 2017Article
- Article
- Interneuronal Transfer and Distal Action of Tetanus Toxin and Botulinum Neurotoxins A and D in Central Neurons.Cell reports · 2016Article
- An optical biosensor assay for rapid dual detection of Botulinum neurotoxins A and E.Scientific reports · 2015Article
- Recommended Immunological Strategies to Screen for Botulinum Neurotoxin-Containing Samples.Toxins · 2015Article
- Enhanced detection of type C botulinum neurotoxin by the Endopep-MS assay through optimization of peptide substrates.Bioorganic & medicinal chemistry · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Botulinum neurotoxins (BoNTs) are highly toxic proteases produced by anaerobic bacteria. Traditionally, a mouse bioassay (MBA) has been used for detection of BoNTs, but for a long time, laboratories have worked with alternative methods for their detection. One of the most promising in vitro methods is a combination of an enzymatic and mass spectrometric assay called Endopep-MS. However, no comprehensive validation of the method has been presented. The main purpose of this work was to perform a validation for the qualitative analysis of BoNT-A, B, C, C/D, D, D/C, and F in serum. The limit of detection (LOD), selectivity, precision, stability in matrix and solution, and correlation with the MBA were evaluated. The LOD was equal to or even better than that of the MBA for BoNT-A, B, D/C, E, and F. Furthermore, Endopep-MS was for the first time successfully used to differentiate between BoNT-C and D and their mosaics C/D and D/C by different combinations of antibodies and target peptides. In addition, sequential antibody capture was presented as a new way to multiplex the method when only a small sample volume is available. In the comparison with the MBA, all the samples analyzed were positive for BoNT-C/D with both methods. These results indicate that the Endopep-MS method is a valid alternative to the MBA as the gold standard for BoNT detection based on its sensitivity, selectivity, and speed and that it does not require experimental animals.
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