ArticleAntonie van Leeuwenhoek2014
Melanization and morphological effects on antifungal susceptibility of Penicillium marneffei.
Article in Antonie van Leeuwenhoek, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Exploring allomelanin: A comparative analysis via natural product extraction and synthesis.Science advances · 2026Article
- Development and validation of a colorimetric antifungal susceptibility testing method for the dimorphic fungus Talaromyces marneffei.Medical mycology · 2023Article
- Extracellular vesicles derived fromFrontiers in immunology · 2023Article
- Comparison of in vitro Susceptibilities ofInfection and drug resistance · 2023Article
- Transcription Factors Pmr1 and Pmr2 Cooperatively Regulate Melanin Biosynthesis, Conidia Development and Secondary Metabolism inJournal of fungi (Basel, Switzerland) · 2021Article
- Fungal Melanin and the Mammalian Immune System.Journal of fungi (Basel, Switzerland) · 2021Review
- Study of the Effect of Nutrient Environment on the Biological Characteristics ofJournal of ophthalmology · 2021Article
- Characterization of a novel yeast phase-specific antigen expressed during in vitro thermal phase transition of Talaromyces marneffei.Scientific reports · 2020Article
- Melanization of Fusarium keratoplasticum (F. solani Species Complex) During Disseminated Fusariosis in a Patient with Acute Leukemia.Mycopathologia · 2017Article
- Agrobacterium tumefaciens-mediated transformation: an efficient tool for targeted gene disruption in Talaromyces marneffei.World journal of microbiology & biotechnology · 2017Article
- Melanins Protect Sporothrix brasiliensis and Sporothrix schenckii from the Antifungal Effects of Terbinafine.PloS one · 2016Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The biosynthesis of melanin has been linked with virulence in diverse pathogenic fungi. Penicillium marneffei, a dimorphic fungus, is capable of melanization in both mycelial and yeast phases, and the pigment may be produced during infection to protect the fungus from the host immune system. To investigate the impact of yeast morphological transformation on antifungal susceptibility, P. marneffei was cultured on various media including minimal medium, 1 % tryptone, brain heart infusion broth, and malt extract broth by using the standardized susceptibility protocol (the M27-A protocol, RPMI medium) for yeasts. We also investigated whether P. marneffei melanization affected its susceptibility to antifungal drugs by adding L-DOPA into culture broths. There were no differences in the minimum inhibitory concentrations of P. marneffei yeast cells previously grown in various culture broths with or without L-DOPA using the M27A protocol (into which no melanin substrate can be added due to a rapid colour change of the RPMI medium to black) for testing amphotericin B, clotrimazole, fluconazole, itraconazole and ketoconazole. However, both melanized and non-melanized P. marneffei displayed increased resistance to antifungal drugs when L-DOPA was added into a selected assay medium, 0.17 % yeast nitrogen base, 2 % glucose, and 1.5 % agar. Hence, active melanin formation appears to protect P. marneffei by enhancing its resistance to antifungal drugs.
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