ArticleScientific reports2014
On-chip immobilization of planarians for in vivo imaging.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Adaptive robustness through incoherent signaling mechanisms in a regenerative brain.Cell reports · 2024Article
- Neurophysiological measurements of planarian brain activity: a unique model for neuroscience research.Biology open · 2024Article
- Live Imaging in Planarians: Immobilization and Real-Time Visualization of Reactive Oxygen Species.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Direct Current Electric Stimulation Alters the Frequency and the Distribution of Mitotic Cells in Planarians.Bioelectricity · 2021Article
- Bioelectronics for Millimeter-Sized Model Organisms.iScience · 2020Review
- Neural control of body-plan axis in regenerating planaria.PLoS computational biology · 2019Article
- The Role of Early Bioelectric Signals in the Regeneration of Planarian Anterior/Posterior Polarity.Biophysical journal · 2019Article
- Linalool acts as a fast and reversible anesthetic in Hydra.PloS one · 2019Article
- Behavioral Research with Planaria.Perspectives on behavior science · 2018Article
- Mechanics dictate where and how freshwater planarians fission.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Long-Term, Stochastic Editing of Regenerative Anatomy via Targeting Endogenous Bioelectric Gradients.Biophysical journal · 2017Article
- Physiological controls of large-scale patterning in planarian regeneration: a molecular and computational perspective on growth and form.Regeneration (Oxford, England) · 2016Article
- Gap Junctional Blockade Stochastically Induces Different Species-Specific Head Anatomies in Genetically Wild-Type Girardia dorotocephala Flatworms.International journal of molecular sciences · 2015Article
- Bioelectrical regulation of cell cycle and the planarian model system.Biochimica et biophysica acta · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Planarians are an important model organism for regeneration and stem cell research. A complete understanding of stem cell and regeneration dynamics in these animals requires time-lapse imaging in vivo, which has been difficult to achieve due to a lack of tissue-specific markers and the strong negative phototaxis of planarians. We have developed the Planarian Immobilization Chip (PIC) for rapid, stable immobilization of planarians for in vivo imaging without injury or biochemical alteration. The chip is easy and inexpensive to fabricate, and worms can be mounted for and removed after imaging within minutes. We show that the PIC enables significantly higher-stability immobilization than can be achieved with standard techniques, allowing for imaging of planarians at sub-cellular resolution in vivo using brightfield and fluorescence microscopy. We validate the performance of the PIC by performing time-lapse imaging of planarian wound closure and sequential imaging over days of head regeneration. We further show that the device can be used to immobilize Hydra, another photophobic regenerative model organism. The simple fabrication, low cost, ease of use, and enhanced specimen stability of the PIC should enable its broad application to in vivo studies of stem cell and regeneration dynamics in planarians and Hydra.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.