Evidence map›Paper›PMID 25226390›Full record

ArticlePloS one2014

Non-invasive Drosophila ECG recording by using eutectic gallium-indium alloy electrode: a feasible tool for future research on the molecular mechanisms involved in cardiac arrhythmia.

Po-Hung Kuo, Te-Hsuen Tzeng, Yi-Chun Huang, Yu-Hao Chen, Yi-Chung Chang, Yi-Lwun Ho, June-Tai Wu, Hsiu-Hsian Lee, Po-Jung Lai, Kwei-Yan Liu and 2 more

Open access · goldAbstract read
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In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Probing the Electrophysiology of the Developing Heart.Journal of cardiovascular development and disease · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Po-Hung KuoGraduate Institute of Electronics Engineering, National Taiwan University, Taipei, Taiwan.
Te-Hsuen TzengGraduate Institute of Electronics Engineering, National Taiwan University, Taipei, Taiwan.
Yi-Chun HuangGraduate Institute of Biomedical Electronics and Informatics, National Taiwan University, Taipei, Taiwan.
Yu-Hao ChenGraduate Institute of Electronics Engineering, National Taiwan University, Taipei, Taiwan.
Yi-Chung ChangCenter for Dynamical Biomarkers and Translation Medicine, National Central University, Taoyuan, Taiwan; and Research Center for Adaptive Data Analysis, National Central University, Taoyuan, Taiwan.
Yi-Lwun HoSection of Cardiology, Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
June-Tai WuInstitute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Hsiu-Hsian LeeInstitute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Po-Jung LaiInstitute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Kwei-Yan LiuInstitute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Ya-Chen ChengInstitute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Shey-Shi LuGraduate Institute of Electronics Engineering, National Taiwan University, Taipei, Taiwan.
National Taiwan University · TWNational Central University · TWNational Taiwan University Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDrosophila heart tube is a feasible model for cardiac physiological research. However, obtaining Drosophila electrocardiograms (ECGs) is difficult, due to the weak signals and limited contact area to apply electrodes. This paper presents a non-invasive Gallium-Indium (GaIn) based recording system for Drosophila ECG measurement, providing the heart rate and heartbeat features to be observed. This novel, high-signal-quality system prolongs the recording time of insect ECGs, and provides a feasible platform for research on the molecular mechanisms involved in cardiovascular diseases.

methodsIn this study, two types of electrode, tungsten needle probes and GaIn electrodes, were used respectively to noiselessly conduct invasive and noninvasive ECG recordings of Drosophila. To further analyze electrode properties, circuit models were established and simulated. By using electromagnetic shielded heart signal acquiring system, consisted of analog amplification and digital filtering, the ECG signals of three phenotypes that have different heart functions were recorded without dissection. RESULTS AND DISCUSSION: The ECG waveforms of different phenotypes of Drosophila recorded invasively and repeatedly with n value (n>5) performed obvious difference in heart rate. In long period ECG recordings, non-invasive method implemented by GaIn electrodes acts relatively stable in both amplitude and period. To analyze GaIn electrode, the correctness of GaIn electrode model established by this paper was validated, presenting accuracy, stability, and reliability.

conclusionsNoninvasive ECG recording by GaIn electrodes was presented for recording Drosophila pupae ECG signals within a limited contact area and signal strength. Thus, the observation of ECG changes in normal and SERCA-depleted Drosophila over an extended period is feasible. This method prolongs insect survival time while conserving major ECG features, and provides a platform for electrophysiological signal research on the molecular mechanism involved in cardiac arrhythmia, as well as research related to drug screening and development.

Indexed as

DrosophilaElectrocardiographyElectrodesAnimalsArrhythmias, CardiacResearch

Identifiers

PMID25226390
PMCPMC4165757
OpenAlexW2029937205

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.