ArticleACS synthetic biology2015
Native promoter strategy for high-yielding synthesis and engineering of fungal secondary metabolites.
Article in ACS synthetic biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
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- Decoding Pecan's Fungal Foe: A Genomic Insight intoJournal of fungi (Basel, Switzerland) · 2025Article
- Deciphering chemical logic of fungal natural product biosynthesis through heterologous expression and genome mining.Natural product reports · 2023Review
- Total Heterologous Biosynthesis of Fungal Natural Products inJournal of natural products · 2022Review
- Recent advances in the chemo-biological characterization of decalin natural products and unraveling of the workings of Diels-Alderases.Fungal biology and biotechnology · 2022Review
- Discovery and investigation of natural Diels-Alderases.Journal of natural medicines · 2021Review
- Catalytic mechanism andNature catalysis · 2021Article
- Applications of CRISPR/Cas9 in the Synthesis of Secondary Metabolites in Filamentous Fungi.Frontiers in microbiology · 2021Review
- Article
- Advances in linking polyketides and non-ribosomal peptides to their biosynthetic gene clusters in Fusarium.Current genetics · 2019Review
- Synthetic control devices for gene regulation in Penicillium chrysogenum.Microbial cell factories · 2019Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Secondary Metabolites of Onygenales Fungi Exemplified by Aioliomyces pyridodomos.Journal of natural products · 2019Article
- Technology development for natural product biosynthesis in Saccharomyces cerevisiae.Current opinion in biotechnology · 2016Review
- Recent advances of Diels-Alderases involved in natural product biosynthesis.The Journal of antibiotics · 2016Review
- Synthetic biology of fungal natural products.Frontiers in microbiology · 2015Review
- Filamentous fungi from extreme environments as a promising source of novel bioactive secondary metabolites.Frontiers in microbiology · 2015Article
- Isolation of pyrrolocins A-C: cis- and trans-decalin tetramic acid antibiotics from an endophytic fungal-derived pathway.Journal of natural products · 2014Article
- Development and optimization of an intergeneric conjugation system and analysis of promoter activity in Streptomyces rimosus M527.Journal of Zhejiang University. Science. BArticle
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Strategies are needed for the robust production of cryptic, silenced, or engineered secondary metabolites in fungi. The filamentous fungus Fusarium heterosporum natively synthesizes the polyketide equisetin at >2 g L(-1) in a controllable manner. We hypothesized that this production level was achieved by regulatory elements in the equisetin pathway, leading to the prediction that the same regulatory elements would be useful in producing other secondary metabolites. This was tested by using the native eqxS promoter and eqxR regulator in F. heterosporum, synthesizing heterologous natural products in yields of ∼1 g L(-1). As proof of concept for the practical application, we resurrected an extinct pathway from an endophytic fungus with an initial yield of >800 mg L(-1), leading to the practical synthesis of a selective antituberculosis agent. Finally, the method enabled new insights into the function of polyketide synthases in filamentous fungi. These results demonstrate a strategy for optimally employing native regulators for the robust synthesis of secondary metabolites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.