ArticleJournal of visualized experiments : JoVE2014
A microfluidic technique to probe cell deformability.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Article
- Article
- A constriction channel analysis of astrocytoma stiffness and disease progression.Biomicrofluidics · 2021Article
- Stress hormone signaling through β-adrenergic receptors regulates macrophage mechanotype and function.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Integrated automated particle tracking microfluidic enables high-throughput cell deformability cytometry for red cell disorders.American journal of hematology · 2019Article
- Characterizing Deformability of Drug Resistant Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells Using Acoustic Tweezers.Scientific reports · 2018Article
- Microfluidic generation of transient cell volume exchange for convectively driven intracellular delivery of large macromolecules.Materials today (Kidlington, England) · 2018Article
- Article
- Quantitative Deformability Cytometry: Rapid, Calibrated Measurements of Cell Mechanical Properties.Biophysical journal · 2017Article
- Cancer cells become less deformable and more invasive with activation of β-adrenergic signaling.Journal of cell science · 2016Article
- Stiffness of pancreatic cancer cells is associated with increased invasive potential.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Tumour-suppressor microRNAs regulate ovarian cancer cell physical properties and invasive behaviour.Open biology · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we detail the design, fabrication, and use of a microfluidic device to evaluate the deformability of a large number of individual cells in an efficient manner. Typically, data for ~10(2) cells can be acquired within a 1 hr experiment. An automated image analysis program enables efficient post-experiment analysis of image data, enabling processing to be complete within a few hours. Our device geometry is unique in that cells must deform through a series of micron-scale constrictions, thereby enabling the initial deformation and time-dependent relaxation of individual cells to be assayed. The applicability of this method to human promyelocytic leukemia (HL-60) cells is demonstrated. Driving cells to deform through micron-scale constrictions using pressure-driven flow, we observe that human promyelocytic (HL-60) cells momentarily occlude the first constriction for a median time of 9.3 msec before passaging more quickly through the subsequent constrictions with a median transit time of 4.0 msec per constriction. By contrast, all-trans retinoic acid-treated (neutrophil-type) HL-60 cells occlude the first constriction for only 4.3 msec before passaging through the subsequent constrictions with a median transit time of 3.3 msec. This method can provide insight into the viscoelastic nature of cells, and ultimately reveal the molecular origins of this behavior.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.