ArticleJournal of visualized experiments : JoVE2014
A method for microinjection of Patiria miniata zygotes.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Regeneration of the larval sea star nervous system by wounding induced respecification to the Sox2 lineage.eLife · 2022Article
- Live imaging of echinoderm embryos to illuminate evo-devo.Frontiers in cell and developmental biology · 2022Article
- The Development and Neuronal Complexity of Bipinnaria Larvae of the Sea Star Asterias rubens.Integrative and comparative biology · 2021Article
- Review
- Systematic comparison of sea urchin and sea star developmental gene regulatory networks explains how novelty is incorporated in early development.Nature communications · 2020Article
- Analysis of sea star larval regeneration reveals conserved processes of whole-body regeneration across the metazoa.BMC biology · 2019Article
- Embryo microinjection of the lecithotrophic sea urchinJournal of biological methods · 2019Article
- A cdk1 gradient guides surface contraction waves in oocytes.Nature communications · 2017Article
- Genome-wide use of high- and low-affinity Tbrain transcription factor binding sites during echinoderm development.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Establishment of molecular genetic approaches to study gene expression and function in an invasive hemipteran,EvoDevo · 2017Article
- Dose-dependent nuclear β-catenin response segregates endomesoderm along the sea star primary axis.Development (Cambridge, England) · 2015Article
- Modular evolution of DNA-binding preference of a Tbrain transcription factor provides a mechanism for modifying gene regulatory networks.Molecular biology and evolution · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Echinoderms have long been a favorite model system for studies of reproduction and development, and more recently for the study of gene regulation and evolution of developmental processes. The sea star, Patiria miniata, is gaining prevalence as a model system for these types of studies which were previously performed almost exclusively in the sea urchins, Strongylocentrotus purpuratus and Lytechinus variegatus. An advantage of these model systems is the ease of producing modified embryos in which a particular gene is up or downregulated, labeling a group of cells, or introducing a reporter gene. A single microinjection method is capable of creating a wide variety of such modified embryos. Here, we present a method for obtaining gametes from P. miniata, producing zygotes, and introducing perturbing reagents via microinjection. Healthy morphant embryos are subsequently isolated for quantitative and qualitative studies of gene function. The availability of genome and transcriptome data for this organism has increased the types of studies that are performed and the ease of executing them.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.