ArticlePLoS biology2014
A trihelix DNA binding protein counterbalances hypoxia-responsive transcriptional activation in Arabidopsis.
Article in PLoS biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 118 citations in OpenAlex.
- Distinct processes contributing to post-submergence recovery determine leaf age-specific flood resilience in Arabidopsis.Plant physiology · 2026Article
- HNature · 2026Article
- The SnRK1-RAP2.4h-PIP2 module contributes to the trade-off between growth and hypoxia tolerance in plants.Nature communications · 2026Article
- The next frontier: Exploring plant hypoxia sensing and response mechanisms through synthetic biology.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cross-Regulation of Metabolic and Immune Pathways in Plants Under Hypoxic Conditions.Plants (Basel, Switzerland) · 2026Review
- A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Plastid-to-nucleus communication under hypoxia involves group VII ethylene response factors inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Trihelix transcription factors: versatile regulators in plant growth and development, abiotic, and biotic stress responses.Plant cell reports · 2026Review
- Interaction Between CsATG8f and CsRAP2.12 Modulates Antioxidant Defense and Hypoxia Response During Submergence inInternational journal of molecular sciences · 2025Article
- An overview of low-oxygen sensing and flooding responses of tomato.Journal of experimental botany · 2025Review
- Genome-wide identification and expression analysis of trihelix transcription factor family in cucumber (Cucumis sativus L.) and their roles in biotic stress responses.BMC genomics · 2025Article
- Beware of Sealing Film of Petri Dishes!-Alters the Expression of a Large Number of Genes.International journal of molecular sciences · 2025Article
- Reciprocal modulation of responses to nitrate starvation and hypoxia in roots and leaves ofPlant signaling & behavior · 2024Article
- The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation.Plant physiology · 2024Article
- Primed to persevere: Hypoxia regulation from epigenome to protein accumulation in plants.Plant physiology · 2024Review
- Transcriptomics of long-term, low oxygen storage coupled with ethylene signaling interference suggests neofunctionalization of hypoxia response pathways in apple (Plant direct · 2024Article
- Whole-Genome Sequencing and Analysis of Tumour-Forming Radish (International journal of molecular sciences · 2024Article
- Coordination between two cis-elements of WRKY33, bound by the same transcription factor, confers humid adaption in Arabidopsis thaliana.Plant molecular biology · 2024Article
- Group VII ethylene response factors forming distinct regulatory loops mediate submergence responses.Plant physiology · 2024Article
- Plant responses to limited aeration: Advances and future challenges.Plant direct · 2023Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional activation in response to hypoxia in plants is orchestrated by ethylene-responsive factor group VII (ERF-VII) transcription factors, which are stable during hypoxia but destabilized during normoxia through their targeting to the N-end rule pathway of selective proteolysis. Whereas the conditionally expressed ERF-VII genes enable effective flooding survival strategies in rice, the constitutive accumulation of N-end-rule-insensitive versions of the Arabidopsis thaliana ERF-VII factor RAP2.12 is maladaptive. This suggests that transcriptional activation under hypoxia that leads to anaerobic metabolism may need to be fine-tuned. However, it is presently unknown whether a counterbalance of RAP2.12 exists. Genome-wide transcriptome analyses identified an uncharacterized trihelix transcription factor gene, which we named HYPOXIA RESPONSE ATTENUATOR1 (HRA1), as highly up-regulated by hypoxia. HRA1 counteracts the induction of core low oxygen-responsive genes and transcriptional activation of hypoxia-responsive promoters by RAP2.12. By yeast-two-hybrid assays and chromatin immunoprecipitation we demonstrated that HRA1 interacts with the RAP2.12 protein but with only a few genomic DNA regions from hypoxia-regulated genes, indicating that HRA1 modulates RAP2.12 through protein-protein interaction. Comparison of the low oxygen response of tissues characterized by different levels of metabolic hypoxia (i.e., the shoot apical zone versus mature rosette leaves) revealed that the antagonistic interplay between RAP2.12 and HRA1 enables a flexible response to fluctuating hypoxia and is of importance to stress survival. In Arabidopsis, an effective low oxygen-sensing response requires RAP2.12 stabilization followed by HRA1 induction to modulate the extent of the anaerobic response by negative feedback regulation of RAP2.12. This mechanism is crucial for plant survival under suboptimal oxygenation conditions. The discovery of the feedback loop regulating the oxygen-sensing mechanism in plants opens new perspectives for breeding flood-resistant crops.
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