ArticleJournal of visualized experiments : JoVE2014
Collection and extraction of saliva DNA for next generation sequencing.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Nitrate-responsive oral microbiome modulates nitric oxide homeostasis and blood pressure in humans.Free radical biology & medicine · 2018Trial
- The potential bactericidal effect of oral probiotics on extrinsic black stain-associated bacteria in children: AnJournal of dental sciences · 2025Article
- Candidate genes related to spiritual mediumship: a whole-exome sequencing analysis of highly gifted mediums.Revista brasileira de psiquiatria (Sao Paulo, Brazil : 1999) · 2025Article
- Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5-9.Clinical and experimental dental research · 2024Article
- Oxytocin Receptor Single-Nucleotide Polymorphisms Are Related to Maternal-Infant Co-Occupation and Infant Sensory Processing.Children (Basel, Switzerland) · 2024Article
- Dopamine and Serotonin Transporter Genes Regulation in Highly Sensitive Individuals during Stressful Conditions: A Focus on Genetics and Epigenetics.Biomedicines · 2024Article
- A Genetic Tool to Identify Predators Responsible for Livestock Attacks in South America and Recommendations for Human-Wildlife Conflict Mitigation.Animals : an open access journal from MDPI · 2024Article
- Mild internet use is associated with epigenetic alterations of key neurotransmission genes in salivary DNA of young university students.Scientific reports · 2023Article
- Validation of RT-qPCR test for SARS-CoV-2 in saliva specimens.Journal of infection and public health · 2022Article
- Preanalytical DNA assessment for downstream applications: How to optimize the management of human biospecimens to support molecular diagnosis-An experimental study.Journal of clinical laboratory analysis · 2022Article
- Regulation of oxytocin receptor gene expression in obsessive-compulsive disorder: a possible role for the microbiota-host epigenetic axis.Clinical epigenetics · 2022Article
- OXTR Gene DNA Methylation Levels Are Associated with Discounting Behavior with Untrustworthy Proposers.Brain sciences · 2022Article
- Article
- The Use of Saliva as a Biosample in the Light of COVID-19.Diagnostics (Basel, Switzerland) · 2021Review
- Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA.Scientific reports · 2020Article
- Technical and Methodological Aspects of Cell-Free Nucleic Acids Analyzes.International journal of molecular sciences · 2020Review
- New COVID-19 saliva-based test: How good is it compared with the current nasopharyngeal or throat swab test?Journal of the Chinese Medical Association : JCMA · 2020Review
- A Novel Approach for the Identification of Pharmacogenetic Variants inJournal of clinical medicine · 2020Article
- Specimen Collection for Translational Studies in Hidradenitis Suppurativa.Scientific reports · 2019Article
- A comparison of genetic sampling methodologies for candidate-gene analyses.Journal of translational science · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The preferred source of DNA in human genetics research is blood, or cell lines derived from blood, as these sources yield large quantities of high quality DNA. However, DNA extraction from saliva can yield high quality DNA with little to no degradation/fragmentation that is suitable for a variety of DNA assays without the expense of a phlebotomist and can even be acquired through the mail. However, at present, no saliva DNA collection/extraction protocols for next generation sequencing have been presented in the literature. This protocol optimizes parameters of saliva collection/storage and DNA extraction to be of sufficient quality and quantity for DNA assays with the highest standards, including microarray genotyping and next generation sequencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.