ArticleJournal of visualized experiments : JoVE2014
Using plusTipTracker software to measure microtubule dynamics in Xenopus laevis growth cones.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
- Dual spatio-temporal regulation of axon growth and microtubule dynamics by RhoA signaling pathways.Journal of cell science · 2024Article
- Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Live Imaging of Cytoskeletal Dynamics in EmbryonicCold Spring Harbor protocols · 2021Article
- The +TIP Navigator-1 is an actin-microtubule crosslinker that regulates axonal growth cone motility.The Journal of cell biology · 2020Article
- Mitotic Motor KIFC1 Is an Organizer of Microtubules in the Axon.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- XMAP215 promotes microtubule-F-actin interactions to regulate growth cone microtubules during axon guidance inJournal of cell science · 2019Article
- Characterization of Xenopus laevis guanine deaminase reveals new insights for its expression and function in the embryonic kidney.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- Proscillaridin A exerts anti-tumor effects through GSK3β activation and alteration of microtubule dynamics in glioblastoma.Cell death & disease · 2018Article
- Neuronal-Specific TUBB3 Is Not Required for Normal Neuronal Function but Is Essential for Timely Axon Regeneration.Cell reports · 2018Article
- The Microtubule-Associated Protein Tau Mediates the Organization of Microtubules and Their Dynamic Exploration of Actin-Rich Lamellipodia and Filopodia of Cortical Growth Cones.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Neuronal Growth Cone Size-Dependent and -Independent Parameters of Microtubule Polymerization.Frontiers in cellular neuroscience · 2018Article
- Neurotoxic mechanisms of paclitaxel are local to the distal axon and independent of transport defects.Experimental neurology · 2017Article
- Xenopus laevis as a model system to study cytoskeletal dynamics during axon pathfinding.Genesis (New York, N.Y. : 2000) · 2017Review
- Xenopus TACC2 is a microtubule plus end-tracking protein that can promote microtubule polymerization during embryonic development.Molecular biology of the cell · 2016Article
- High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells.Journal of visualized experiments : JoVE · 2016Article
- Conserved roles for cytoskeletal components in determining laterality.Integrative biology : quantitative biosciences from nano to macro · 2016Review
- Using Xenopus laevis retinal and spinal neurons to study mechanisms of axon guidance in vivo and in vitro.Seminars in cell & developmental biology · 2016Review
- Xenopus TACC1 is a microtubule plus-end tracking protein that can regulate microtubule dynamics during embryonic development.Cytoskeleton (Hoboken, N.J.) · 2015Article
- TIPsy tour guides: how microtubule plus-end tracking proteins (+TIPs) facilitate axon guidance.Frontiers in cellular neuroscience · 2015Review
- TACC3 is a microtubule plus end-tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types.Molecular biology of the cell · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Microtubule (MT) plus-end-tracking proteins (+TIPs) localize to the growing plus-ends of MTs and regulate MT dynamics(1,2). One of the most well-known and widely-utilized +TIPs for analyzing MT dynamics is the End-Binding protein, EB1, which binds all growing MT plus-ends, and thus, is a marker for MT polymerization(1). Many studies of EB1 behavior within growth cones have used time-consuming and biased computer-assisted, hand-tracking methods to analyze individual MTs(1-3). Our approach is to quantify global parameters of MT dynamics using the software package, plusTipTracker(4), following the acquisition of high-resolution, live images of tagged EB1 in cultured embryonic growth cones(5). This software is a MATLAB-based, open-source, user-friendly package that combines automated detection, tracking, visualization, and analysis for movies of fluorescently-labeled +TIPs. Here, we present the protocol for using plusTipTracker for the analysis of fluorescently-labeled +TIP comets in cultured Xenopus laevis growth cones. However, this software can also be used to characterize MT dynamics in various cell types(6-8).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.