ArticleJournal of visualized experiments : JoVE2014
Rapid isolation and purification of mitochondria for transplantation by tissue dissociation and differential filtration.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04998357 (Study Title), which is not on this map. Cited by 78 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Study Title: Autologous Mitochondrial Transplant for Cerebral Ischemia
Who cites it
78 citing papers in PubMed, 129 citations in OpenAlex.
- Mitochondrial transplantation in lung diseases: From mechanisms to application prospects.Genes & diseases · 2026Review
- Therapeutic effect of mitochondrial transfer on bone tissue diseases: treatment strategy of mitochondrial transplantation and delivery technology.Journal of orthopaedic translation · 2026Review
- Article
- Mitochondrial Transplantation Therapy for Ischemic Stroke: Progress and Challenges.Cellular and molecular neurobiology · 2026Review
- Mitochondria transplantation mitigates attenuation of muscle fiber regeneration by evoked contractions.American journal of physiology. Cell physiology · 2026Article
- Assessment of mitochondrial viability under calcium Stress: Insights for mitochondrial transplantation.Mitochondrion · 2026Article
- Rethinking mitochondrial isolation for the next generation of mitochondrial therapies.Frontiers in physiology · 2026Review
- Mitochondrial Transplantation Increases Bioenergetics and Neurite Outgrowth in Healthy and P301Ltau-Expressing SH-SY5Y Cells.Molecular neurobiology · 2025Article
- Human dental pulp stem cell-derived mitochondria restore mitochondrial function and promote neuroregeneration in a cellular model of Parkinson's disease.Stem cell research & therapy · 2025Article
- Feasibility of Xenogeneic Mitochondrial Transplantation in Neuronal Systems: An Exploratory Study.Life (Basel, Switzerland) · 2025Article
- Mitochondrial transplantation combined with mitoquinone and melatonin: A survival strategy against myocardial reperfusion injury in aged rats.Experimental physiology · 2025Article
- Transplantation of exogenous mitochondria mitigates myocardial dysfunction after cardiac arrest.eLife · 2025Article
- The role of homogenization cycles and Poloxamer 188 on the quality of mitochondria isolated for use in mitochondrial transplantation therapy.Scientific reports · 2025Article
- Article
- Mitotherapy attenuates sepsis-induced brain injury in rats subjected to cecal ligation and puncture: Role of SIRT-1/PGC-1α network.Iranian journal of basic medical sciences · 2025Article
- Recent advances in mitochondrial transplantation to treat disease.Biomaterials translational · 2025Review
- A method for isolating and cryopreserving intact mitochondria with improved integrity and functionality.Biophysics and physicobiology · 2025Article
- Mitochondrial Transplantation: A Paradigm Shift in Osteoporosis Therapy.International journal of nanomedicine · 2025Review
- Mitochondrial transplantation normalizes transcriptomic and proteomic shift associated with ischemia reperfusion injury in neonatal hearts donated after circulatory death.Scientific reports · 2024Article
- Mechanism and prospects of mitochondrial transplantation for spinal cord injury treatment.Stem cell research & therapy · 2024Review
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Previously described mitochondrial isolation methods using differential centrifugation and/or Ficoll gradient centrifugation require 60 to 100 min to complete. We describe a method for the rapid isolation of mitochondria from mammalian biopsies using a commercial tissue dissociator and differential filtration. In this protocol, manual homogenization is replaced with the tissue dissociator's standardized homogenization cycle. This allows for uniform and consistent homogenization of tissue that is not easily achieved with manual homogenization. Following tissue dissociation, the homogenate is filtered through nylon mesh filters, which eliminate repetitive centrifugation steps. As a result, mitochondrial isolation can be performed in less than 30 min. This isolation protocol yields approximately 2 x 10(10) viable and respiration competent mitochondria from 0.18 ± 0.04 g (wet weight) tissue sample.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.