Evidence map›Paper›PMID 25225522›Full record

ArticlePakistan journal of medical sciences2014

Induction of B7-H1 expression by human cytomegalovirus in extravillous cytotrophoblast cells and role of MAPK pathway.

Wenrong Gong, Jianhua Zhao, Zhen Chen, Lin Lei, Lihua Luo, Xuehong Zhao, Hui Xing, Suhua Chen, Qisheng Tu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pakistan journal of medical sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 85% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Wenrong GongWenrong Gong, MD, Center for Molecular Medicine of Medical College/ Central Laboratory of the Affiliated Hospital, Hubei University of Arts and Science, Xiang Yang, Hubei, China 441053.
Jianhua ZhaoJianhua Zhao, MD, Department of Obstetrics and Gynaecology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430030, China.
Zhen ChenZhen Chen, MD, Department of Ophthalmology, Renmin Hospital, Wuhan University, Wuhan 430060, China.
Lin LeiLin Lei, MD,Department of Oncology, The Affiliated Hospital of Hubei University of Arts and Science, Xiang Yang, Hubei, China 441021.
Lihua LuoLihua Luo, MD, Center for Molecular Medicine of Medical College/ Central Laboratory of the Affiliated Hospital, Hubei University of Arts and Science, Xiang Yang, Hubei, China 441053.
Xuehong ZhaoXuehong Zhao, MD, Center for Molecular Medicine of Medical College/ Central Laboratory of the Affiliated Hospital, Hubei University of Arts and Science, Xiang Yang, Hubei, China 441053.
Hui XingHui Xing, MD, Department of Obstetrics and Gynaecology, The Affiliated Hospital of Hubei University of Arts and Science, Xiang Yang, Hubei, China 441021.
Suhua ChenSuhua Chen, MD, Department of Obstetrics and Gynaecology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, 430030, China.
Qisheng TuQisheng Tu, MD, Oral Biology, Tufts University School of Dental Medicine, 1 Kneeland Street, Boston, MA 02111, USA.
Tongji Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis paper is aimed at to evaluate B7-H1 expression as induced by human cytomegalovirus (HCMV) in extravillous cytotrophoblast cell line HPT-8 and possible underlying mechanism.

methodReal time PCR and flow cytometry were used to determine B7-H1 mRNA and protein before and after HCMV infection in HPT-8 cells. Western blot analysis was used to determine the level of MAPK phosphorylation in HPT-8 cell lines infected with HCMV.

results100TCID50 was found to be the most effective dose, capable of stimulating B7-H1 mRNA and protein expression in HPT-8 cells. When empty control group was considered to have a B7-H1 mRNA value of 1, B7-H1 mRNA was 4.32 in 100TCID50 group. In flow cytometry study, mean fluorescence intensity (MFI) of 100TCID50 group was 16.14, while empty control group was 1.34. Both mRNA and protein expression were found to be significantly increased (P<0.05) in 100TCID50 group compared to empty control group. The result of Western blot analysis showed increase in B7-H1 expression caused by the extracellular signaling that was related to ERK activation and the ERK inhibitor U0126 was found to reverse this increase.

conclusionHCMV upregulates B7-H1 expression in human extravillous cytotrophoblast cell line HPT-8, which is related to MAPK activation. Our result would be helpful in finding better therapies against intrauterine HCMV infection.

Indexed as

B7-H1CytomegalovirusCytotrophoblastMAPK

Identifiers

PMID25225522
PMCPMC4163228
OpenAlexW2312510242

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.