ArticleProceedings of the National Academy of Sciences of the United States of America2014
LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- Article
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- Nanoplastics-mediated physiologic and genomic responses in pathogenic Escherichia coli O157:H7.Journal of nanobiotechnology · 2025Article
- FliA regulates the antibacterial activity of plantaricin BM-1 againstFrontiers in microbiology · 2025Article
- Phenotypic and transcriptomic analysis reveals the antibiofilm mechanism ofCurrent research in food science · 2025Article
- Less is more: the lack of autoinducer-2-dependent quorum sensing promotes competitive fitness ofFrontiers in cellular and infection microbiology · 2025Article
- Quorum sensing regulators and non-ribosomal peptide synthetases govern antibacterial secretions inFrontiers in microbiology · 2025Article
- Metabolic activities of marine ammonia-oxidizing archaea orchestrated by quorum sensing.mLife · 2024Article
- Spatial structure, chemotaxis and quorum sensing shape bacterial biomass accumulation in complex porous media.Nature communications · 2024Article
- GraphSite: Ligand Binding Site Classification with Deep Graph Learning.Biomolecules · 2022Article
- Mechanisms of Inhibition of Quorum Sensing as an Alternative for the Control ofMicroorganisms · 2022Review
- Structural Characterization of LsrK as a Quorum Sensing Target and a Comparison between X-ray and Homology Models.Journal of chemical information and modeling · 2021Article
- An Efficient Synthesis of Optically Active [4-Molecules (Basel, Switzerland) · 2021Article
- A Redox-Based Autoinduction Strategy to Facilitate Expression of 5xCys-Tagged Proteins for Electrobiofabrication.Frontiers in microbiology · 2021Article
- Tackling Antimicrobial Resistance with Small Molecules Targeting LsrK: Challenges and Opportunities.Journal of medicinal chemistry · 2020Article
- CRISPR-Pathogens (Basel, Switzerland) · 2020Article
- Lsr operon is associated with AI-2 transfer and pathogenicity in avian pathogenic Escherichia coli.Veterinary research · 2019Article
- Quorum Sensing and Metabolic State of the Host Control Lysogeny-Lysis Switch of Bacteriophage T1.mBio · 2019Article
- Identification of novel autoinducer-2 receptors inThe Journal of biological chemistry · 2019Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
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Abstract
The quorum sensing signal autoinducer-2 (AI-2) regulates important bacterial behaviors, including biofilm formation and the production of virulence factors. Some bacteria, such as Escherichia coli, can quench the AI-2 signal produced by a variety of species present in the environment, and thus can influence AI-2-dependent bacterial behaviors. This process involves uptake of AI-2 via the Lsr transporter, followed by phosphorylation and consequent intracellular sequestration. Here we determine the metabolic fate of intracellular AI-2 by characterizing LsrF, the terminal protein in the Lsr AI-2 processing pathway. We identify the substrates of LsrF as 3-hydroxy-2,4-pentadione-5-phosphate (P-HPD, an isomer of AI-2-phosphate) and coenzyme A, determine the crystal structure of an LsrF catalytic mutant bound to P-HPD, and identify the reaction products. We show that LsrF catalyzes the transfer of an acetyl group from P-HPD to coenzyme A yielding dihydroxyacetone phosphate and acetyl-CoA, two key central metabolites. We further propose that LsrF, despite strong structural homology to aldolases, acts as a thiolase, an activity previously undescribed for this family of enzymes. With this work, we have fully characterized the biological pathway for AI-2 processing in E. coli, a pathway that can be used to quench AI-2 and control quorum-sensing-regulated bacterial behaviors.
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