ArticleProceedings of the National Academy of Sciences of the United States of America2014
Both endo-siRNAs and tRNA-derived small RNAs are involved in the differentiation of primitive eukaryote Giardia lamblia.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 51 citations in OpenAlex.
- Review
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- A thermo-resistant and RNase-sensitive cargo fromJournal of extracellular biology · 2023Article
- The Exosome-like Vesicles ofInternational journal of molecular sciences · 2023Article
- Advances in Protozoan Epigenetic Targets and Their Inhibitors for the Development of New Potential Drugs.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Small and intermediate size structural RNAs in the unicellular parasiteMicrobial genomics · 2022Article
- SARS-CoV-2 causes a significant stress response mediated by small RNAs in the blood of COVID-19 patients.Molecular therapy. Nucleic acids · 2022Article
- Review
- AASRA: an anchor alignment-based small RNA annotation pipeline†.Biology of reproduction · 2021Article
- PANDORA-seq expands the repertoire of regulatory small RNAs by overcoming RNA modifications.Nature cell biology · 2021Article
- tsRBase: a comprehensive database for expression and function of tsRNAs in multiple species.Nucleic acids research · 2021Article
- Noncoding RNAs in Apicomplexan Parasites: An Update.Trends in parasitology · 2020Review
- Giardia lamblia miRNAs as a new diagnostic tool for human giardiasis.PLoS neglected tropical diseases · 2019Article
- Metazoan tsRNAs: Biogenesis, Evolution and Regulatory Functions.Non-coding RNA · 2019Review
- Genome-wide identification and characterization of transfer RNA-derived small RNAs in Plasmodium falciparum.Parasites & vectors · 2019Article
- miRNA-seq ofFrontiers in microbiology · 2019Article
- SPORTS1.0: A Tool for Annotating and Profiling Non-coding RNAs Optimized for rRNA- and tRNA-derived Small RNAs.Genomics, proteomics & bioinformatics · 2018Article
- MINTmap: fast and exhaustive profiling of nuclear and mitochondrial tRNA fragments from short RNA-seq data.Scientific reports · 2017Article
- Computational Approaches to tRNA-Derived Small RNAs.Non-coding RNA · 2017Review
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small RNAs (sRNAs), including microRNAs and endogenous siRNAs (endo-siRNAs), regulate most important biologic processes in eukaryotes, such as cell division and differentiation. Although sRNAs have been extensively studied in various eukaryotes, the role of sRNAs in the early emergence of eukaryotes is unclear. To address these questions, we deep sequenced the sRNA transcriptome of four different stages in the differentiation of Giardia lamblia, one of the most primitive eukaryotes. We identified a large number of endo-siRNAs in this fascinating parasitic protozoan and found that they were produced from live telomeric retrotransposons and three genomic regions (i.e., endo-siRNA generating regions [eSGRs]). eSGR-derived endo-siRNAs were proven to target mRNAs in trans. Gradual up-regulation of endo-siRNAs in the differentiation of Giardia suggested that they might be involved in the regulation of this process. This hypothesis was supported by the impairment of the differentiation ability of Giardia when GLDICER, essential for the biogenesis of endo-siRNAs, was knocked down. Endo-siRNAs are not the only sRNA regulators in Giardia differentiation, because a great number of tRNAs-derived sRNAs showed more dramatic expression changes than endo-siRNAs in this process. We totally identified five novel kinds of tRNAs-derived sRNAs and found that the biogenesis in four of them might be correlated with that of stress-induced tRNA-derived RNA (sitRNA), which was discovered in our previous studies. Our studies reveal an unexpected complex panorama of sRNA in G. lamblia and shed light on the origin and functional evolution of eukaryotic sRNAs.
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