ArticleMolecular and cellular biology2014
Direct single-stranded DNA binding by Teb1 mediates the recruitment of Tetrahymena thermophila telomerase to telomeres.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- RPA-like single-stranded DNA-binding protein complexes including CST serve as specialized processivity factors for polymerases.Current opinion in structural biology · 2023Review
- Orchestrating nucleic acid-protein interactions at chromosome ends: telomerase mechanisms come into focus.Nature structural & molecular biology · 2023Review
- Structural basis of human telomerase recruitment by TPP1-POT1.Science (New York, N.Y.) · 2022Article
- Disruption of a ∼23-24 nucleotide small RNA pathway elevates DNA damage responses inMolecular biology of the cell · 2021Article
- Cryo-EM structures tell a tale of two telomerases.Nature structural & molecular biology · 2021Article
- Article
- Spatial constraints on chromosomes are instrumental to meiotic pairing.Journal of cell science · 2020Article
- Structural biology of telomerase and its interaction at telomeres.Current opinion in structural biology · 2017Review
- Telomerase Mechanism of Telomere Synthesis.Annual review of biochemistry · 2017Review
- Progress in Human and Tetrahymena Telomerase Structure Determination.Annual review of biophysics · 2017Review
- Shared Subunits of Tetrahymena Telomerase Holoenzyme and Replication Protein A Have Different Functions in Different Cellular Complexes.The Journal of biological chemistry · 2017Article
- Evolution of Arabidopsis protection of telomeres 1 alters nucleic acid recognition and telomerase regulation.Nucleic acids research · 2016Article
- Integrative structural biology of Tetrahymena telomerase - insights into catalytic mechanism and interaction at telomeres.The FEBS journal · 2016Review
- The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex.Nature structural & molecular biology · 2015Article
- Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions.Science (New York, N.Y.) · 2015Article
- Dynamics of Human Telomerase Holoenzyme Assembly and Subunit Exchange across the Cell Cycle.The Journal of biological chemistry · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The eukaryotic reverse transcriptase telomerase copies its internal RNA template to synthesize telomeric DNA repeats at chromosome ends in balance with sequence loss during cell proliferation. Previous work has established several factors involved in telomerase recruitment to telomeres in yeast and mammalian cells; however, it remains unclear what determines the association of telomerase with telomeres in other organisms. Here we investigate the cell cycle dependence of telomere binding by each of the seven Tetrahymena thermophila telomerase holoenzyme proteins TERT, p65, Teb1, p50, p75, p45, and p19. We observed coordinate cell cycle-regulated recruitment and release of all of the subunits, including the telomeric-repeat DNA-binding subunit Teb1. Using domain truncation and mutagenesis approaches, we investigated which subunits govern the interaction of telomerase holoenzyme with telomeres. Our results show that Teb1 is critical for telomere interaction of other holoenzyme subunits and demonstrate that high-affinity Teb1 DNA-binding activity is necessary and sufficient for cell cycle-regulated telomere association. Overall, these and additional findings indicate that in the ciliate Tetrahymena, telomerase recruitment to telomeres requires direct binding to single-stranded DNA, unlike the indirect DNA recognition through telomere-bound proteins essential in yeast and mammalian cells.
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