ArticleThe Journal of reproduction and development2014
Prolonged exposure to hyaluronidase decreases the fertilization and development rates of fresh and cryopreserved mouse oocytes.
Article in The Journal of reproduction and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Integration of Bioengineered Tools in Assisted Reproductive Technologies.Advanced healthcare materials · 2025Review
- Development of a low-cost microfluidic chip for hyaluronidase-free oocyte denudation in mammals.Biotechnology notes (Amsterdam, Netherlands) · 2025Article
- A study on the effect of the number of expansion units in a microfluidic chip on hyaluronidase-free oocyte denudation in mammals.Journal of electrical bioimpedance · 2025Article
- Comprehensive atlas of mitochondrial distribution and dynamics during oocyte maturation in mouse models.Biomarker research · 2024Article
- Design and implementation of a lab-on-a-chip for assisted reproductive technologies.BioImpacts : BI · 2024Article
- Toxicity of the recombinant human hyaluronidase ALT-BC4 on embryonic development.Journal of animal science and technology · 2021Article
- The role of extracellular matrix in normal and pathological pregnancy: Future applications of microphysiological systems in reproductive medicine.Experimental biology and medicine (Maywood, N.J.) · 2020Review
- Cryopreservation of mouse resources.Laboratory animal research · 2020Review
- Graphene Oxide ImprovesFrontiers in bioengineering and biotechnology · 2020Article
- N-acetyl cysteine restores the fertility of vitrified-warmed mouse oocytes derived through ultrasuperovulation.PloS one · 2019Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hyaluronidase is generally used to remove cumulus cells from mouse oocytes before oocyte cryopreservation, intracytoplasmic sperm injection or DNA injection. In general, use of cumulus-free mouse oocytes decreases in vitro fertilizing ability compared with cumulus-surrounded oocytes. The effect of hyaluronidase exposure on the quality of mouse oocytes is not fully understood. Here, we investigated the effect of hyaluronidase exposure time on the fertilization rate of fresh and vitrified mouse oocytes and their subsequent developmental ability in vitro. We found that the fertilization rate decreased with hyaluronidase treatments. This reduction in the fertilization rate following treatment with hyaluronidase was fully reversed by removal of the zona pellucida. In addition, oocytes treated with hyaluronidase for 5 min or longer had a reduced capacity to develop to the morula and blastocyst stage. The survival, fertilization, and developmental rates of vitrified-warmed oocytes were also reduced by longer exposure to hyaluronidase. In conclusion, these results suggest that prolonged exposure to hyaluronidase decreases the quality of mouse oocytes and shorter hyaluronidase treatment times may help achieve a stable and high fertilization rate in fresh and cryopreserved oocytes.
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