ArticleThe Journal of cell biology1989
Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
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The trial behind it
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Who cites it
79 citing papers in PubMed, 258 citations in OpenAlex.
- Article
- Axonal transport of Frizzled5 by Alcadein α-containing vesicles is associated with kinesin-1.Molecular biology of the cell · 2023Article
- CRISPR-Cas9 Knock-In of T513M and G41S Mutations in the Murine β-Galactosyl-Ceramidase Gene Re-capitulates Early-Onset and Adult-Onset Forms of Krabbe Disease.Frontiers in molecular neuroscience · 2022Article
- Kinesin-1 activity recorded in living cells with a precipitating dye.Nature communications · 2021Article
- Defined Tau Phosphospecies Differentially Inhibit Fast Axonal Transport Through Activation of Two Independent Signaling Pathways.Frontiers in molecular neuroscience · 2020Article
- Development of cone photoreceptors and their synapses in the human and monkey fovea.The Journal of comparative neurology · 2019Article
- Herpes Simplex Virus, Alzheimer's Disease and a Possible Role for Rab GTPases.Frontiers in cell and developmental biology · 2019Article
- Fluorescence microscopy applied to intracellular transport by microtubule motors.Journal of biosciences · 2018Review
- Transglutaminase and polyamination of tubulin: posttranslational modification for stabilizing axonal microtubules.Neuron · 2013Article
- Quantitative measurements and modeling of cargo-motor interactions during fast transport in the living axon.Physical biology · 2012Article
- WAVE2 Protein Complex Coupled to Membrane and Microtubules.Journal of oncology · 2012Article
- Kinesin-73 is a processive motor that localizes to Rab5-containing organelles.The Journal of biological chemistry · 2011Article
- Gamma-diketone axonopathy: analyses of cytoskeletal motors and highways in CNS myelinated axons.Toxicological sciences : an official journal of the Society of Toxicology · 2010Article
- APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Conventional kinesin holoenzymes are composed of heavy and light chain homodimers.Biochemistry · 2008Article
- Kidins220/ARMS is transported by a kinesin-1-based mechanism likely to be involved in neuronal differentiation.Molecular biology of the cell · 2007Article
- Axonal transport, amyloid precursor protein, kinesin-1, and the processing apparatus: revisited.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- A novel CDK5-dependent pathway for regulating GSK3 activity and kinesin-driven motility in neurons.The EMBO journal · 2004Article
- The Kinesin-related protein Costal2 associates with membranes in a Hedgehog-sensitive, Smoothened-independent manner.The Journal of biological chemistry · 2004Article
- Reduction of detyrosinated microtubules and Golgi fragmentation are linked to tau-induced degeneration in astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Kinesin, a microtubule-activated ATPase and putative motor protein for the transport of membrane-bounded organelles along microtubules, was purified from bovine brain and used as an immunogen for the production of murine monoclonal antibodies. Hybridoma lines that secreted five distinct antikinesin IgGs were cloned. Three of the antibodies reacted on immunoblots with the 124-kD heavy chain of kinesin, while the other two antibodies recognized the 64-kD light chain. When used for immunofluorescence microscopy, the antibodies stained punctate, cytoplasmic structures in a variety of cultured mammalian cell types. Consistent with the identification of these structures as membrane-bounded organelles was the observation that cells which had been extracted with Triton X-100 before fixation contained little or no immunoreactive material. Staining of microtubules in the interphase cytoplasm or mitotic spindle was never observed, nor were associated structures, such as centrosomes and primary cilia, labeled by any of the antibodies. Nevertheless, in double-labeling experiments using antibodies to kinesin and tubulin, kinesin-containing particles were most abundant in regions where microtubules were most highly concentrated and the particles often appeared to be aligned on microtubules. These results constitute the first direct evidence for the association of kinesin with membrane-bounded organelles, and suggest a molecular mechanism for organelle motility based on transient interactions of organelle-bound kinesin with the microtubule surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.