ArticleBMC plant biology2014
GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots.
Article in BMC plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 54 citations in OpenAlex.
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- Construction and Validation of CRISPR/Cas Vectors for Editing theCurrent issues in molecular biology · 2024Article
- Functional analysis of root-preferential oil palm metallothionein promoter in tobacco.Transgenic research · 2024Article
- A Brief Review of Plant Cell Transfection, Gene Transcript Expression, and Genotypic Integration for Enhancing Compound Production.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Characterization of two constitutive promotersaBIOTECH · 2022Article
- Identification of an anther-specific promoter from a male sterile AB line in Chinese cabbage (3 Biotech · 2022Article
- Enhancing plant growth and biomass production by overexpression of GA20ox gene under control of a root preferential promoter.Transgenic research · 2022Article
- Analysis of expression characteristics of soybean leaf and root tissue-specific promoters in Arabidopsis and soybean.Transgenic research · 2021Article
- A 1232 bp upstream sequence of glutamine synthetase 1b from Eichhornia crassipes is a root-preferential promoter sequence.BMC plant biology · 2021Article
- A Golgi-Localized Sodium/Hydrogen Exchanger Positively Regulates Salt Tolerance by Maintaining Higher KFrontiers in plant science · 2021Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Genetic engineering of parthenocarpic tomato plants using transient SlIAA9 knockdown by novel tissue-specific promoters.Scientific reports · 2019Article
- The ABA-induced soybean ERF transcription factor gene GmERF75 plays a role in enhancing osmotic stress tolerance in Arabidopsis and soybean.BMC plant biology · 2019Article
- Cell Wall Proteins Play Critical Roles in Plant Adaptation to Phosphorus Deficiency.International journal of molecular sciences · 2019Review
- The Roles ofFrontiers in plant science · 2019Article
- Identification and characterization ofPeerJ · 2019Article
- Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (International journal of molecular sciences · 2018Article
- Double genetically modified symbiotic system for improved Cu phytostabilization in legume roots.Environmental science and pollution research international · 2017Article
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6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundPromoters play important roles in gene expression and function. There are three basic types of promoters: constitutive, specific, and inducible. Constitutive promoters are widely used in genetic engineering, but these promoters have limitations. Inducible promoters are activated by specific inducers. Tissue-specific promoters are a type of specific promoters that drive gene expression in specific tissues or organs. Here, we cloned and characterized the GmPRP2 promoter from soybean. The expression pattern indicated that this promoter is root-preferential in transgenic Arabidopsis and the hairy roots of soybean. It can be used to improve the root resistance or tolerance to pathogens, pests, malnutrition and other abiotic stresses which cause extensive annual losses in soybean production.
resultsThe GmPRP2 promoter (GmPRP2p-1062) was isolated from soybean cv. Williams 82. Sequence analysis revealed that this promoter contains many cis-acting elements, including root-specific motifs. The GmPRP2p-1062 and its 5'-deletion fragments were fused with the GUS reporter gene and introduced into Arabidopsis and the hairy roots of soybean to further determine promoter activity. Histochemical analysis in transgenic Arabidopsis showed that GUS activity was mainly detected in roots and hypocotyls in all deletion fragments except GmPRP2p-471 (a 5'-deletion fragment of GmPRP2p-1062 with 471 bp length). GUS activity was higher in transgenic Arabidopsis and hairy roots with GmPRP2p-1062 and GmPRP2p-852 (a 5'-deletion fragment of GmPRP2p-1062 with 852 bp length) constructs than the other two constructs. GUS activity was enhanced by NaCl, PEG, IAA and JM treatments and decreased by SA, ABA and GA treatments in transgenic Arabidopsis.
conclusionsGmPRP2p-1062 is a root-preferential promoter, and its core fragment for root-preferential expression might lie between -369 and +1. GmPRP2p-852 may be useful in the genetic engineering of novel soybean cultivars in the future.
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