Evidence map›Paper›PMID 25224340›Full record

ArticleExperimental eye research2014

Inner retinal preservation in rat models of retinal degeneration implanted with subretinal photovoltaic arrays.

Jacob G Light, James W Fransen, Adewumi N Adekunle, Alice Adkins, Gobinda Pangeni, James Loudin, Keith Mathieson, Daniel V Palanker, Maureen A McCall, Machelle T Pardue

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Using CRISPR-Cas9 to Generate Gene-Corrected Autologous iPSCs for the Treatment of Inherited Retinal Degeneration.Molecular therapy : the journal of the American Society of Gene Therapy · 2017
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Jacob G LightOphthalmology, Emory University, USA; Rehab R&D Center of Excellence, Atlanta VA Medical Center, USA.
James W FransenAnatomical Sciences & Neurobiology, University of Louisville, USA.
Adewumi N AdekunleOphthalmology, Emory University, USA.
Alice AdkinsRehab R&D Center of Excellence, Atlanta VA Medical Center, USA.
Gobinda PangeniOphthalmology & Visual Sciences, University of Louisville, USA.
James LoudinHansen Experimental Physics Laboratory, Stanford University, USA.
Keith MathiesonHansen Experimental Physics Laboratory, Stanford University, USA; Institute of Photonics, University of Strathclyde, UK.
Daniel V PalankerHansen Experimental Physics Laboratory, Stanford University, USA; Ophthalmology, Stanford University, USA.
Maureen A McCallAnatomical Sciences & Neurobiology, University of Louisville, USA; Ophthalmology & Visual Sciences, University of Louisville, USA.
Machelle T PardueOphthalmology, Emory University, USA; Rehab R&D Center of Excellence, Atlanta VA Medical Center, USA. Electronic address: mpardue@emory.edu.
Emory University · USUniversity of Louisville · USStanford University · USAtlanta VA Medical Center · USUniversity of Strathclyde · GB

Funding

Spectrum Stanford Center for clinical and Translational Research and EducationUL1TR001085 · NCATS · STANFORD UNIVERSITY · PI CULLEN, MARK RICHARD, GREENBERG, HARRY BERNARD · 2013 to 2017
$37.1M
Stanford Center for Clinical and Translational Education and Research (SCCTER)UL1UL1RR025744 · NCRR · STANFORD UNIVERSITY · PI GREENBERG, HARRY BERNARD · 2008 to 2011
$22.4M
High Resolution Photovoltaic Retinal ProsthesisR01EY018608 · NEI · STANFORD UNIVERSITY · PI PALANKER, DANIEL V · 2009 to 2016
$9.2M
Training Program in TransplantationT32HL076138 · NHLBI · UNIVERSITY OF LOUISVILLE · PI ILDSTAD, SUZANNE T, SHIRWAN, HAVAL · 2004 to 2013
$2.4M
NCATS NIH HHS UL1 TR001085NCRR NIH HHS UL1 RR025744NEI NIH HHS R01 EY018608NEI NIH HHS R01-EY018608NHLBI NIH HHS 5 T32 HL 76138-09NHLBI NIH HHS T32 HL076138
6 · The paper itself

Abstract

Photovoltaic arrays (PVA) implanted into the subretinal space of patients with retinitis pigmentosa (RP) are designed to electrically stimulate the remaining inner retinal circuitry in response to incident light, thereby recreating a visual signal when photoreceptor function declines or is lost. Preservation of inner retinal circuitry is critical to the fidelity of this transmitted signal to ganglion cells and beyond to higher visual targets. Post-implantation loss of retinal interneurons or excessive glial scarring could diminish and/or eliminate PVA-evoked signal transmission. As such, assessing the morphology of the inner retina in RP animal models with subretinal PVAs is an important step in defining biocompatibility and predicting success of signal transmission. In this study, we used immunohistochemical methods to qualitatively and quantitatively compare inner retinal morphology after the implantation of a PVA in two RP models: the Royal College of Surgeons (RCS) or transgenic S334ter-line 3 (S334ter-3) rhodopsin mutant rat. Two PVA designs were compared. In the RCS rat, we implanted devices in the subretinal space at 4 weeks of age and histologically examined them at 8 weeks of age and found inner retinal morphology preservation with both PVA devices. In the S334ter-3 rat, we implanted devices at 6-12 weeks of age and again, inner retinal morphology was generally preserved with either PVA design 16-26 weeks post-implantation. Specifically, the length of rod bipolar cells and numbers of cholinergic amacrine cells were maintained along with their characteristic inner plexiform lamination patterns. Throughout the implanted retinas we found nonspecific glial reaction, but none showed additional glial scarring at the implant site. Our results indicate that subretinally implanted PVAs are well-tolerated in rodent RP models and that the inner retinal circuitry is preserved, consistent with our published results showing implant-evoked signal transmission.

Indexed as

Disease Models, AnimalVisual ProsthesisAmacrine CellsAnimalsBiomarkersElectroretinographyEpendymoglial CellsFluorescent Antibody Technique, IndirectGlial Fibrillary Acidic ProteinProsthesis ImplantationProtein Kinase C-alphaRatsRats, Mutant StrainsRats, TransgenicRetinal Bipolar CellsRetinitis PigmentosaBiomarkersGlial Fibrillary Acidic ProteinProtein Kinase C-alphaAmacrine cellsBipolar cellsMüller glial cellsProstheticRetina

Identifiers

PMID25224340
PMCPMC4566954
OpenAlexW1984564072

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.