ArticleBMC plant biology2014
Positive Darwinian selection is a driving force for the diversification of terpenoid biosynthesis in the genus Oryza.
Article in BMC plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 39 citations in OpenAlex.
- Genomic mechanism of aroma terpenoids biosynthesis in plants.Journal of advanced research · 2026Review
- Expansion of the geranylgeranyl pyrophosphate synthase gene family underlies the evolution of terpenoid biosynthesis in termites.PLoS biology · 2026Article
- Integrative omics reveals mechanisms of biosynthesis and regulation of floral scent in Cymbidium tracyanum.Plant biotechnology journal · 2025Article
- Allelic variation of terpene synthases drives terpene diversity in the wild species of the Freesia genus.Plant physiology · 2023Article
- Genome-wide identification, expression profile and evolutionary relationships of TPS genes in the neotropical fruit tree species Psidium cattleyanum.Scientific reports · 2023Article
- Transcriptome sequencing and gas chromatography-mass spectrometry analyses provide insights into β-caryophyllene biosynthesis inFood chemistry. Molecular sciences · 2022Article
- Evolution and co-evolution: insights into the divergence of plant heat shock factor genes.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Single-cell RNA-sequencing of Nicotiana attenuata corolla cells reveals the biosynthetic pathway of a floral scent.The New phytologist · 2022Article
- Evolution of Terpene Synthases in Orchidaceae.International journal of molecular sciences · 2021Review
- Protective Effects of (Nutrients · 2020Review
- Evolution of isoprenyl diphosphate synthase-like terpene synthases in fungi.Scientific reports · 2020Article
- The reconstruction and biochemical characterization of ancestral genes furnish insights into the evolution of terpene synthase function in the Poaceae.Plant molecular biology · 2020Article
- Diverse Terpenoids and Their Associated Antifungal Properties from Roots of Different Cultivars ofMolecules (Basel, Switzerland) · 2020Article
- The evolution of metabolism: How to test evolutionary hypotheses at the genomic level.Computational and structural biotechnology journal · 2020Review
- Combinatorial Evolution of a Terpene Synthase Gene Cluster Explains Terpene Variations inPlant physiology · 2020Article
- Identification and functional characterization of three new terpene synthase genes involved in chemical defense and abiotic stresses in Santalum album.BMC plant biology · 2019Article
- Terpene Biosynthesis in Red Algae Is Catalyzed by Microbial Type But Not Typical Plant Terpene Synthases.Plant physiology · 2019Article
- Biosynthesis and Emission of Stress-Induced Volatile Terpenes in Roots and Leaves of Switchgrass (Frontiers in plant science · 2019Article
- Transcriptomic and metabolic flux analyses reveal shift of metabolic patterns during rice grain development.BMC systems biology · 2018Article
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6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTerpenoids constitute the largest class of secondary metabolites made by plants and display vast chemical diversity among and within species. Terpene synthases (TPSs) are the pivotal enzymes for terpenoid biosynthesis that create the basic carbon skeletons of this class. Functional divergence of paralogous and orthologous TPS genes is a major mechanism for the diversification of terpenoid biosynthesis. However, little is known about the evolutionary forces that have shaped the evolution of plant TPS genes leading to terpenoid diversity.
resultsThe orthologs of Oryza Terpene Synthase 1 (OryzaTPS1), a rice terpene synthase gene involved in indirect defense against insects in Oryza sativa, were cloned from six additional Oryza species. In vitro biochemical analysis showed that the enzymes encoded by these OryzaTPS1 genes functioned either as (E)-β-caryophyllene synthases (ECS), or (E)-β-caryophyllene & germacrene A synthases (EGS), or germacrene D & germacrene A synthases (DAS). Because the orthologs of OryzaTPS1 in maize and sorghum function as ECS, the ECS activity was inferred to be ancestral. Molecular evolutionary detected the signature of positive Darwinian selection in five codon substitutions in the evolution from ECS to DAS. Homology-based structure modeling and the biochemical analysis of laboratory-generated protein variants validated the contribution of the five positively selected sites to functional divergence of OryzaTPS1. The changes in the in vitro product spectra of OryzaTPS1 proteins also correlated closely to the changes in in vivo blends of volatile terpenes released from insect-damaged rice plants.
conclusionsIn this study, we found that positive Darwinian selection is a driving force for the functional divergence of OryzaTPS1. This finding suggests that the diverged sesquiterpene blend produced by the Oryza species containing DAS may be adaptive, likely in the attraction of the natural enemies of insect herbivores.
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