ReviewThe Journal of antimicrobial chemotherapy2015
Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination.
Review in The Journal of antimicrobial chemotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 87 citations in OpenAlex.
- An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how?Parasitology · 2020Pooled it
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- Sensitive near point-of-care detection of asymptomatic and submicroscopic Plasmodium falciparum infections in African endemic countries.Nature communications · 2025Article
- Advancing artemisinin resistance monitoring using a high sensitivity ddPCR assay for Pfkelch13 mutation detection in Asia.Scientific reports · 2025Article
- Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.3 Biotech · 2023Review
- Assessing afebrile malaria and bed-net use in a high-burden region of India: Findings from multiple rounds of mass screening.PloS one · 2023Article
- Detection ofPathogens (Basel, Switzerland) · 2022Article
- Plasmodium knowlesi: the game changer for malaria eradication.Malaria journal · 2022Review
- Current methods for the detection ofCurrent research in parasitology & vector-borne diseases · 2022Review
- RPAcan3990: an Ultrasensitive Recombinase Polymerase Assay To DetectJournal of clinical microbiology · 2021Article
- Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings.Diagnostics (Basel, Switzerland) · 2021Review
- Review
- Evaluation of Malaria Diagnostic Methods as a Key for Successful Control and Elimination Programs.Tropical medicine and infectious disease · 2020Review
- Rapid diagnostic tests failing to detect infections by Plasmodium falciparum encoding pfhrp2 and pfhrp3 genes in a non-endemic setting.Malaria journal · 2020Article
- Detection of malaria parasites in dried human blood spots using mid-infrared spectroscopy and logistic regression analysis.Malaria journal · 2019Article
- Use cases for genetic epidemiology in malaria elimination.Malaria journal · 2019Review
- Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil.Malaria journal · 2019Article
- A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos.PloS one · 2019Article
- Poor Diagnostic Performance of a Species-Specific Loop-Mediated Isothermal Amplification (LAMP) Platform for Malaria.European journal of microbiology & immunology · 2018Article
- Potential Point-of-Care Testing for Dengue Virus in the Field.Journal of clinical microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
In countries where malaria transmission has decreased substantially, thanks to the scale-up of control interventions, malaria elimination may be feasible. Nevertheless, this goal requires new strategies such as the active detection and treatment of infected individuals. As the detection threshold for the currently used diagnostic methods is 100 parasites/μL, most low-density, asymptomatic infections able to maintain transmission cannot be detected. Identifying them by molecular methods such as PCR is a possible option but the field deployment of these tests is problematic. Isothermal amplification of nucleic acids (at a constant temperature) offers the opportunity of addressing some of the challenges related to the field deployment of molecular diagnostic methods. One of the novel isothermal amplification methods for which a substantial amount of work has been done is the loop-mediated isothermal amplification (LAMP) assay. The present review describes LAMP and several other isothermal nucleic acid amplification methods, such as thermophilic helicase-dependent amplification, strand displacement amplification, recombinase polymerase amplification and nucleic acid sequence-based amplification, and explores their potential use as high-throughput, field-based molecular tests for malaria diagnosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.