ArticleGenes & development2014
Drosophila TRF2 is a preferential core promoter regulator.
Article in Genes & development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 50 citations in OpenAlex.
- High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo.Research square · 2026Article
- High-resolution binding data of TFIID and cofactors show promoter-specific differencesbioRxiv : the preprint server for biology · 2025Article
- Characterization of core promoter activation by the Drosophila insulator-binding protein BEAF.Scientific reports · 2025Article
- Drosophila architectural proteins M1BP and Opbp cooperatively form the active promoter of a ribosomal protein gene.Epigenetics & chromatin · 2025Article
- TBP facilitates RNA Polymerase I transcription following mitosis.RNA biology · 2024Article
- Functional implications of paralog genes in polyglutamine spinocerebellar ataxias.Human genetics · 2023Review
- Article
- The SAGA core module is critical during Drosophila oogenesis and is broadly recruited to promoters.PLoS genetics · 2021Article
- Computational identification and experimental characterization of preferred downstream positions in human core promoters.PLoS computational biology · 2021Article
- The Core Promoter Is a Regulatory Hub for Developmental Gene Expression.Frontiers in cell and developmental biology · 2021Review
- TBP-Related Factor 2 as a Trigger for Robertsonian Translocations and Speciation.International journal of molecular sciences · 2020Article
- The mode of action of Fruitless: Is it an easy matter to switch the sex?Genes, brain, and behavior · 2020Review
- TAF1 plays a critical role in AML1-ETO driven leukemogenesis.Nature communications · 2019Article
- The RNA Polymerase II Core Promoter inGenetics · 2019Review
- Functional Comparison of Short and Long Isoforms of the TRF2 Protein in Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2019Article
- Distinct gene-selective roles for a network of core promoter factors inBiology open · 2019Article
- TLP-mediated global transcriptional repression after double-strand DNA breaks slows down DNA repair and induces apoptosis.Scientific reports · 2019Article
- Article
- The core-promoter factor TRF2 mediates a Fruitless action to masculinize neurobehavioral traits in Drosophila.Nature communications · 2017Article
- A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes.Nucleic acids research · 2017Article
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 2 countries.
Funding
Abstract
Transcription of protein-coding genes is highly dependent on the RNA polymerase II core promoter. Core promoters, generally defined as the regions that direct transcription initiation, consist of functional core promoter motifs (such as the TATA-box, initiator [Inr], and downstream core promoter element [DPE]) that confer specific properties to the core promoter. The known basal transcription factors that support TATA-dependent transcription are insufficient for in vitro transcription of DPE-dependent promoters. In search of a transcription factor that supports DPE-dependent transcription, we used a biochemical complementation approach and identified the Drosophila TBP (TATA-box-binding protein)-related factor 2 (TRF2) as an enriched factor in the fractions that support DPE-dependent transcription. We demonstrate that the short TRF2 isoform preferentially activates DPE-dependent promoters. DNA microarray analysis reveals the enrichment of DPE promoters among short TRF2 up-regulated genes. Using primer extension analysis and reporter assays, we show the importance of the DPE in transcriptional regulation of TRF2 target genes. It was previously shown that, unlike TBP, TRF2 fails to bind DNA containing TATA-boxes. Using microfluidic affinity analysis, we discovered that short TRF2-bound DNA oligos are enriched for Inr and DPE motifs. Taken together, our findings highlight the role of short TRF2 as a preferential core promoter regulator.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.