ArticleJournal of translational medicine2014
Counter-flow elutriation of clinical peripheral blood mononuclear cell concentrates for the production of dendritic and T cell therapies.
Article in Journal of translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Soluble tissue factor generated by necroptosis-triggered shedding is responsible for thrombosis.Cell research · 2025Article
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- Label-free microfluidic isolation of functional and viable lymphocytes from peripheral blood mononuclear cells.Biomicrofluidics · 2023Article
- Cellular RNA and DNA sensing pathways are essential for the dose-dependent response of human monocytes to ionizing radiation.Frontiers in immunology · 2023Article
- Establishment of a cell processing laboratory to support hematopoietic stem cell transplantation and chimeric antigen receptor (CAR)-T cell therapy.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2021Article
- Adding vitamin C to hydrocortisone lacks benefit in septic shock: a historical cohort study.Canadian journal of anaesthesia = Journal canadien d'anesthesie · 2020Observational
- Recent Advances in Good Manufacturing Practice-Grade Generation of Dendritic Cells.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2020Review
- Past, Present, and Future of Affinity-based Cell Separation Technologies.Acta biomaterialia · 2020Review
- A Molecular Signature in Blood Reveals a Role for p53 in Regulating Malaria-Induced Inflammation.Immunity · 2019Article
- Overcoming Challenges in Process Development of Cellular Therapies.Current hematologic malignancy reports · 2019Review
- Article
- Industrializing Autologous Adoptive Immunotherapies: Manufacturing Advances and Challenges.Frontiers in medicine · 2018Review
- Considerations in T Cell Therapy Product Development for B Cell Leukemia and Lymphoma Immunotherapy.Current hematologic malignancy reports · 2017Review
- Elutriated lymphocytes for manufacturing chimeric antigen receptor T cells.Journal of translational medicine · 2017Article
- The macrophage marker translocator protein (TSPO) is down-regulated on pro-inflammatory 'M1' human macrophages.PloS one · 2017Article
- Myeloid cells in peripheral blood mononuclear cell concentrates inhibit the expansion of chimeric antigen receptor T cells.Cytotherapy · 2016Article
- CAR-T Cell Therapies From the Transfusion Medicine Perspective.Transfusion medicine reviews · 2016Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
introductionPeripheral blood mononuclear cells (PBMC) concentrates collected by apheresis are frequently used as starting material for cellular therapies, but the cell of interest must often be isolated prior to initiating manufacturing. STUDY DESIGN AND
methodsThe results of enriching 59 clinical PBMC concentrates for monocytes or lymphocytes from patients with solid tumors or multiple myeloma using a commercial closed system semi-automated counter-flow elutriation instrument (Elutra, Terumo BCT) were evaluated for quality and consistency. Elutriated monocytes (n = 35) were used to manufacture autologous dendritic cells and elutriated lymphocytes (n = 24) were used manufacture autologous T cell therapies. Elutriated monocytes with >10% neutrophils were subjected to density gradient sedimentation to reduce neutrophil contamination and elutriated lymphocytes to RBC lysis.
resultsElutriation separated the PBMC concentrates into 5 fractions. Almost all of the lymphocytes, platelets and red cells were found in fractions 1 and 2; in contrast, most of the monocytes, 88.6 ± 43.0%, and neutrophils, 74.8 ± 64.3%, were in fraction 5. In addition, elutriation of 6 PBMCs resulted in relatively large quantities of monocytes in fractions 1 or 2. These 6 PBMCs contained greater quantities of monocytes than the other 53 PBMCs. Among fraction 5 isolates 38 of 59 contained >10% neutrophils. High neutrophil content of fraction 5 was associated with greater quantities of neutrophils in the PBMC concentrate. Following density gradient separation the neutrophil counts fell to 3.6 ± 3.4% (all products contained <10% neutrophils). Following red cell lysis of the elutriated lymphocyte fraction the lymphocyte recovery was 86.7 ± 24.0% and 34.3 ± 37.4% of red blood cells remained.
conclusionsElutriation was consistent and effective for isolating monocytes and lymphocytes from PBMC concentrates for manufacturing clinical cell therapies, but further processing is often required.
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