ArticleMolecular microbiology2014
Characterization of Pseudomonas aeruginosa LpxT reveals dual positional lipid A kinase activity and co-ordinated control of outer membrane modification.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- Direct interaction between phosphotransferase LpxT and ArnT modulates polymyxin B resistance inMicrobiology spectrum · 2026Article
- The Genetic Background and Culture Medium Only Marginally Affect the In Vitro Evolution ofAntibiotics (Basel, Switzerland) · 2025Article
- A Multimodal System for Lipid A Structural Analysis from a Single Colony.Analytical chemistry · 2024Article
- Ketogenesis promotes tolerance to Pseudomonas aeruginosa pulmonary infection.Cell metabolism · 2023Article
- Structure Determination of Lipid A with Multiple Glycosylation Sites by Tandem MS of Lithium-Adducted Negative Ions.Journal of the American Society for Mass Spectrometry · 2023Article
- LipidA-IDER to Explore the Global Lipid A Repertoire of Drug-Resistant Gram-Negative Bacteria.Analytical chemistry · 2023Article
- Role of Host and Bacterial Lipids inFrontiers in immunology · 2022Review
- Article
- LpxT-Dependent Phosphorylation of Lipid A inFrontiers in microbiology · 2021Article
- Predominant phosphorylation patterns inJournal of lipid research · 2020Article
- Overexpression ofMicroorganisms · 2020Article
- Ultraviolet Photodissociation Mass Spectrometry for Analysis of Biological Molecules.Chemical reviews · 2020Review
- Transcriptomic Basis of Serum Resistance and Virulence Related Traits in XDRFrontiers in microbiology · 2020Article
- Genetic Basis and Physiological Effects of Lipid A Hydroxylation inPathogens (Basel, Switzerland) · 2019Article
- Pushing the envelope: LPS modifications and their consequences.Nature reviews. Microbiology · 2019Review
- Alterations of Metabolic and Lipid Profiles in Polymyxin-Resistant Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2018Article
- Gene expression kinetics governs stimulus-specific decoration of theScience signaling · 2018Article
- Novel coordination of lipopolysaccharide modifications in Vibrio cholerae promotes CAMP resistance.Molecular microbiology · 2017Article
- Rapid and Consistent Evolution of Colistin Resistance in Extensively Drug-Resistant Pseudomonas aeruginosa during Morbidostat Culture.Antimicrobial agents and chemotherapy · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Gram-negative bacteria have evolved modification machinery to promote a dynamic outer membrane in response to a continually fluctuating environment. The kinase LpxT, for example, adds a phosphate group to the lipid A moiety of some Gram-negatives including Escherichia coli and Salmonella enterica. LpxT activity is inhibited under conditions that compromise membrane integrity, resulting instead in the addition of positively charged groups to lipid A that increase membrane stability and provide resistance to cationic antimicrobial peptides. We have now identified a functional lpxT orthologue in P. aeruginosa. LpxTPa has unique enzymatic characteristics, as it is able to phosphorylate P. aeruginosa lipid A at two sites of the molecule. Surprisingly, a previously uncharacterized lipid A 4'-dephospho-1-triphosphate species was detected. LpxTPa activity is inhibited by magnesium independently of lpxTPa transcription. Modulation of LpxTPa activity is influenced by transcription of the lipid A aminoarabinose transferase ArnT, known to be activated in response to limiting magnesium. These results demonstrate a divergent activity of LpxTPa , and suggest the existence of a co-ordinated regulatory mechanism that permits adaptation to a changing environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.