ArticleBioscience reports2014
Comparison of customized spin-column and salt-precipitation finger-prick blood DNA extraction.
Article in Bioscience reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 27 citations in OpenAlex.
- Reducing DNA extraction costs through factorial design for the DNAdvance Kit.BMC research notes · 2024Article
- Handheld Purification-Free Nucleic Acid Testing Device for Point-of-Need Detection of Malaria from Whole Blood.ACS sensors · 2023Article
- Effects of different delivery modes on teaching biomedical science practical skills in higher education during the 2021 pandemic measures.Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology · 2022Article
- Purification of quantum dot-based bioprobes with a salting out strategy.Nanoscale advances · 2022Article
- To Plate or to Simply Unfreeze, That Is the Question for Optimal Plasmid Extraction.Journal of biomolecular techniques : JBT · 2021Article
- High-yield purification of exceptional-quality, single-molecule DNA substrates.Journal of biological methods · 2021Article
- Continuous Microfluidic Purification of DNA Using Magnetophoresis.Micromachines · 2020Article
- Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood.Journal of biomolecular techniques : JBT · 2018Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
gDNA (genomic DNA extraction from blood is a fundamental process in many diagnostic, identification and research applications. Numerous extraction methods have been reported and are available commercially. However, there is insufficient understanding of the impact of chemical buffers on DNA yield from either whole or nucleated blood. Moreover, these commercial kits are often costly, constraining less well-funded laboratories to traditional and more cost-effective salt-precipitation methods. Towards this, we compared a salt-precipitation and a customized cost-effective spin-column-based method, studying the impact of different chemical constituents on the yields. This customized method resulted in a shortening of the extraction process, higher gDNA yields, and more successful PCR amplification of gDNA genes compared with the salt-precipitation method. Optimizing different chemical buffers on whole- and nucleated blood materials further revealed that certain chemicals boosted extractions from whole- but not nucleated blood. These findings may be useful to laboratories that do not have ready access to commercial kits, and improve their nucleic acid extractions from blood economically.
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Registered trials
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