ArticleBioconjugate chemistry2014
Novel strategy to release and tag N-glycans for functional glycomics.
Article in Bioconjugate chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Enhanced experimental set-up for HPLC-based automated synthesis and purification of carbohydrate building blocks.Carbohydrate research · 2025Article
- HPLC-Based Automated Synthesis and Purification of Carbohydrates.Chemistry (Weinheim an der Bergstrasse, Germany) · 2024Article
- Glycan Array Technology.Advances in biochemical engineering/biotechnology · 2021Article
- Preparation of Complex Glycans From Natural Sources for Functional Study.Frontiers in chemistry · 2020Review
- Natural and Synthetic Sialylated Glycan Microarrays and Their Applications.Frontiers in molecular biosciences · 2019Review
- Automated Chemical Oligosaccharide Synthesis: Novel Approach to Traditional Challenges.Chemical reviews · 2018Review
- Immobilization of glycans on solid surfaces for application in glycomics.Journal of carbohydrate chemistry · 2018Article
- Glycomic and glycoproteomic analysis of glycoproteins-a tutorial.Analytical and bioanalytical chemistry · 2017Article
- Recent Advances in the Analysis of Complex Glycoproteins.Analytical chemistry · 2017Review
- Schistosoma mansoni α1,3-fucosyltransferase-F generates the Lewis X antigen.Glycobiology · 2016Article
- Glycan microarrays of fluorescently-tagged natural glycans.Glycoconjugate journal · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Functional glycomics has been impeded by the lack of inexpensive enzymatic and mild chemical methods to acquire natural glycans in significant amounts. In this study, we have developed a new strategy we term "threshing and trimming" (TaT) to quickly obtain N-glycans from glycoproteins and animal tissues. TaT employs low-cost Pronase to degrade peptides and N-bromosuccinimide (NBS) to effect oxidative decarboxylation under very mild reaction conditions to generate homogeneous aglycon moieties as nitriles or aldehydes. These aglycons can be readily conjugated with fluorescent tags for profiling and functional study. TaT is an affordable alternative to expensive specialty enzymes and strong chemical treatment and unpleasant reagents, and should further drive the functional glycomics of N-glycans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.