ArticleJournal of the American Chemical Society2014
Small-molecule labeling of live cell surfaces for three-dimensional super-resolution microscopy.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 131 citations in OpenAlex.
- Single-Molecule Fluorescence Defines a New Dye Chemistry.Chemical & biomedical imaging · 2026Review
- Controlling Intramolecular Rotation with Five-Membered Heterocycles Facilitates the Design of Highly Cell-Permeable Xanthene-Based Fluorogenic Probes.Journal of the American Chemical Society · 2025Article
- Synthetic fluorophores for live-cell fluorescence microscopy and biosensing.Nature chemical biology · 2025Review
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- Photoactivation of BODIPY Fluorescence with Green Light.The Journal of organic chemistry · 2025Article
- Photoswitchable Fluorescent Hydrazone for Super-Resolution Cell Membrane Imaging.Journal of the American Chemical Society · 2025Article
- Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores.bioRxiv : the preprint server for biology · 2024Article
- Recent advances in SERS-based bioanalytical applications: live cell imaging.Nanophotonics (Berlin, Germany) · 2024Review
- The potential of fluorogenicity for single molecule FRET and DyeCycling.QRB discovery · 2024Review
- Photoactivatable BODIPYs for Live-Cell PALM.Molecules (Basel, Switzerland) · 2023Review
- Spirocyclic rhodamine B benzoisothiazole derivative: a multi-stimuli fluorescent switch manifesting ethanol-responsiveness, photo responsiveness, and acidochromism.RSC advances · 2023Article
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- Chiral Motifs in Highly Interpenetrated Metal-Organic Frameworks Formed from Achiral Tetrahedral Ligands.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Surface-enhanced Raman scattering: An emerging tool for sensing cellular function.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022Review
- A sandcastle worm-inspired strategy to functionalize wet hydrogels.Nature communications · 2021Article
- Recent Progress in Small Spirocyclic, Xanthene-Based Fluorescent Probes.Molecules (Basel, Switzerland) · 2020Review
- vLUME: 3D virtual reality for single-molecule localization microscopy.Nature methods · 2020Article
- Tetrazine as a general phototrigger to turn on fluorophores.Chemical science · 2020Article
- Enzymatic Labeling of Bacterial Proteins for Super-resolution Imaging in Live Cells.ACS central science · 2019Article
- Topologically-guided continuous protein crystallization controls bacterial surface layer self-assembly.Nature communications · 2019Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Precise imaging of the cell surface of fluorescently labeled bacteria requires super-resolution methods because the size-scale of these cells is on the order of the diffraction limit. In this work, we present a photocontrollable small-molecule rhodamine spirolactam emitter suitable for non-toxic and specific labeling of the outer surface of cells for three-dimensional (3D) super-resolution (SR) imaging. Conventional rhodamine spirolactams photoswitch to the emitting form with UV light; however, these wavelengths can damage cells. We extended photoswitching to visible wavelengths >400 nm by iterative synthesis and spectroscopic characterization to optimize the substitution on the spirolactam. Further, an N-hydroxysuccinimide-functionalized derivative enabled covalent labeling of amines on the surface of live Caulobacter crescentus cells. Resulting 3D SR reconstructions of the labeled cell surface reveal uniform and specific sampling with thousands of localizations per cell and excellent localization precision in x, y, and z. The distribution of cell stalk lengths (a sub-diffraction-sized cellular structure) was quantified for a mixed population of cells. Pulse-chase experiments identified sites of cell surface growth. Covalent labeling with the optimized rhodamine spirolactam label provides a general strategy to study the surfaces of living cells with high specificity and resolution down to 10-20 nm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.