ReviewFrontiers in genetics2014
Drosophila comet assay: insights, uses, and future perspectives.
Review in Frontiers in genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 44 citations in OpenAlex.
- Tobacco smoke but not e-cigarette vapor induces epithelial barrier disruption, inflammation, and DNA damage in human Calu-3 cells.Scientific reports · 2026Article
- Defining the parameters of comet assay for reliably detecting DNA damage as a marker of X-ray based irradiation in insects treated with non-lethal doses for export fruit sanitation.Pest management science · 2025Article
- Plastic Fly: WhatInternational journal of molecular sciences · 2024Article
- Article
- Measuring DNA modifications with the comet assay: a compendium of protocols.Nature protocols · 2023Review
- Influence of Kv11.1 (hERG1) KPflugers Archiv : European journal of physiology · 2021Article
- An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity.Nature protocols · 2020Article
- In vivo evaluation of the toxicity, genotoxicity, histopathological, and anti-inflammatory effects of the purified bioglycerol byproduct in biodiesel industry.Journal, genetic engineering & biotechnology · 2020Article
- Oral administration of graphene oxide nano-sheets induces oxidative stress, genotoxicity, and behavioral teratogenicity in Drosophila melanogaster.Environmental science and pollution research international · 2019Article
- Drosophila melanogaster as model organism for monitoring and analyzing genotoxicity associated with city air pollution.Environmental science and pollution research international · 2018Article
- Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.Methods in enzymology · 2017Article
- Comet assay as an indirect measure of systemic oxidative stress.Journal of visualized experiments : JoVE · 2015Article
- The comet assay: past, present, and future.Frontiers in genetics · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The comet assay, a very useful tool in genotoxicity and DNA repair testing, is being applied to Drosophila melanogaster since around 15 years ago, by several research groups. This organism is a valuable model for all kind of processes related to human health, including DNA damage response. The assay has been performed mainly in vivo using different larvae cell types (from brain, midgut, hemolymph, and imaginal disk), but also in vitro with the S2 cell line. Since its first application, it has been used to analyze the genotoxicity and action mechanisms of different chemicals, demonstrating good sensitivity and proving its usefulness. Moreover, it is the only assay that can be used to analyze DNA repair in somatic cells in vivo, comparing the effects of chemicals in different repair strains, and to quantitate repair activities in vitro. Additionally, the comet assay in Drosophila, in vivo and in vitro, has been applied to study the influence of protein overexpression on genome integrity and degradation. Although the assay is well established, it could benefit from some research to determine optimal experimental design to standardize it, and then to allow comparisons among laboratories independently of the chosen cell type.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.