ArticleThe Journal of cell biology1989
Differential extractability of influenza virus hemagglutinin during intracellular transport in polarized epithelial cells and nonpolar fibroblasts.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed, 205 citations in OpenAlex.
- Role of lipid rafts in the FGFR2c-mediated oncogenic signaling by involvement of TRPA1 channel in pancreatic ductal adenocarcinoma cells.Cell death & disease · 2026Article
- Review
- Rab3 mediates a pathway for endocytic sorting and plasma membrane recycling of ordered microdomains.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The MAL Protein, an Integral Component of Specialized Membranes, in Normal Cells and Cancer.Cells · 2021Review
- Review
- Virion-Associated Cholesterol Regulates the Infection of Human Parainfluenza Virus Type 3.Viruses · 2019Article
- Neuronal Signaling by Thy-1 in Nanodomains With Specific Ganglioside Composition: Shall We Open the Door to a New Complexity?Frontiers in cell and developmental biology · 2019Article
- Recruitment of mitofusin 2 into "lipid rafts" drives mitochondria fusion induced by Mdivi-1.Oncotarget · 2018Article
- Directional Fluid Transport across Organ-Blood Barriers: Physiology and Cell Biology.Cold Spring Harbor perspectives in biology · 2017Review
- The Continuing Mystery of Lipid Rafts.Journal of molecular biology · 2016Review
- Raft-like membrane domains in pathogenic microorganisms.Current topics in membranes · 2015Review
- Structural and functional analysis of endosomal compartments in epithelial cells.Methods in cell biology · 2015Article
- Intact sphingomyelin biosynthetic pathway is essential for intracellular transport of influenza virus glycoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Formation of raft-like assemblies within clusters of influenza hemagglutinin observed by MD simulations.PLoS computational biology · 2013Article
- Raft-like microdomains play a key role in mitochondrial impairment in lymphoid cells from patients with Huntington's disease.Journal of lipid research · 2012Article
- Polarized sorting and trafficking in epithelial cells.Cell research · 2012Review
- Caveolin 3, flotillin 1 and influenza virus hemagglutinin reside in distinct domains on the sarcolemma of skeletal myofibers.Biochemistry research international · 2012Article
- Membrane organization and lipid rafts.Cold Spring Harbor perspectives in biology · 2011Review
- Association of influenza virus proteins with membrane rafts.Advances in virology · 2011Article
- Cholesterol-binding viral proteins in virus entry and morphogenesis.Sub-cellular biochemistry · 2010Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biochemical changes in the influenza virus hemagglutinin during intracellular transport to the apical plasma membrane of epithelial cells were investigated in Madin-Darby canine kidney (MDCK) cells and in LLC-PK1 cells stably transfected with a hemagglutinin gene. After pulse-labeling a substantial fraction of hemagglutinin was observed to become insoluble in isotonic solutions of Triton X-100. Insolubility of hemagglutinin was detected late in the transport pathway after addition of complex sugars in the Golgi complex but before insertion of the protein in the plasma membrane. Insolubility was not dependent on oligosaccharide modification since deoxymannojirimycin (dMM), which inhibits mannose trimming, failed to prevent its onset. Insolubility was not due to assembly of virus particles at the plasma membrane because insoluble hemagglutinin was also observed in transfected cells. Hemagglutinin insolubility was also seen in MDCK cells cultured in suspension and in chick embryo fibroblasts, indicating that insolubility and plasma membrane polarity are not simply correlated. In addition to insolubility, an apparent transport-dependent reduction of the disulfide bond linking HA1 and HA2 in hemagglutinin was detected. Because of the timing of both insolubility and the loss of the disulfide bond, these modifications may be important in the delivery of the hemagglutinin to the cell surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.