ArticleChemistry & biology2014
A divalent metal ion-dependent N(1)-methyl transfer to G37-tRNA.
Article in Chemistry & biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 31 citations in OpenAlex.
- Nicotinamide-Based Covalent Inhibitors of tRNA (mACS medicinal chemistry letters · 2026Article
- Mechanism of 30S subunit recognition and modification by the conserved bacterial ribosomal RNA methyltransferase RsmI.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mechanistic Insights into SAM-Dependent Methyltransferases: A Review of Computational Approaches.International journal of molecular sciences · 2025Review
- Connecting tRNA Charging and Decoding through the Axis of Nucleotide Modifications at Position 37.Journal of molecular biology · 2025Review
- m6A modification is incorporated into bacterial mRNA without specific functional benefit.Nucleic acids research · 2025Article
- tRNA mbioRxiv : the preprint server for biology · 2023Article
- tRNA methylation resolves codon usage bias at the limit of cell viability.Cell reports · 2022Article
- Tied up in knots: Untangling substrate recognition by the SPOUT methyltransferases.The Journal of biological chemistry · 2022Review
- Structural and functional characterization of TrmM in mProtein science : a publication of the Protein Society · 2022Article
- Loss ofeLife · 2021Article
- MgACS catalysis · 2020Article
- Crystal structure and catalytic mechanism of the essential mRNA (New York, N.Y.) · 2019Article
- tRNA Methylation Is a Global Determinant of Bacterial Multi-drug Resistance.Cell systems · 2019Article
- A Family Divided: Distinct Structural and Mechanistic Features of the SpoU-TrmD (SPOUT) Methyltransferase Superfamily.Biochemistry · 2019Article
- Insights into Catalytic and tRNA Recognition Mechanism of the Dual-Specific tRNA Methyltransferase fromGenes · 2019Article
- Selective terminal methylation of a tRNA wobble base.Nucleic acids research · 2018Article
- Codon-Specific Translation by mFrontiers in genetics · 2018Article
- Mechanistic features of the atypical tRNA m1G9 SPOUT methyltransferase, Trm10.Nucleic acids research · 2017Article
- m1A Post-Transcriptional Modification in tRNAs.Biomolecules · 2017Review
- Mg2+ regulates transcription of mgtA in Salmonella Typhimurium via translation of proline codons during synthesis of the MgtL peptide.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The catalytic mechanism of the majority of S-adenosyl methionine (AdoMet)-dependent methyl transferases requires no divalent metal ions. Here we report that methyl transfer from AdoMet to N(1) of G37-tRNA, catalyzed by the bacterial TrmD enzyme, is strongly dependent on divalent metal ions and that Mg(2+) is the most physiologically relevant. Kinetic isotope analysis, metal rescue, and spectroscopic measurements indicate that Mg(2+) is not involved in substrate binding, but in promoting methyl transfer. On the basis of the pH-activity profile indicating one proton transfer during the TrmD reaction, we propose a catalytic mechanism in which the role of Mg(2+) is to help to increase the nucleophilicity of N(1) of G37 and stabilize the negative developing charge on O(6) during attack on the methyl sulfonium of AdoMet. This work demonstrates how Mg(2+) contributes to the catalysis of AdoMet-dependent methyl transfer in one of the most crucial posttranscriptional modifications to tRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.