ArticleNature methods2014
Nanoparticle vesicle encoding for imaging and tracking cell populations.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Large-scale combinatorial optical barcoding of cells with laser particles.Light, science & applications · 2025Article
- Lipid-Directed Covalent Labeling of Plasma Membranes for Long-Term Imaging, Barcoding and Manipulation of Cells.JACS Au · 2025Article
- High-Throughput Single-Cell Analysis of Nanoparticle-Cell Interactions.Trends in analytical chemistry : TRAC · 2023Article
- Imaging flow cytometry: a primer.Nature reviews. Methods primers · 2022Article
- Wavelength-encoded laser particles for massively multiplexed cell tagging.Nature photonics · 2019Article
- The origin of heterogeneous nanoparticle uptake by cells.Nature communications · 2019Article
- Multiplexed laser particles for spatially resolved single-cell analysis.Light, science & applications · 2019Article
- Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots.Angewandte Chemie (International ed. in English) · 2018Article
- Microscopic inspection and tracking of single upconversion nanoparticles in living cells.Light, science & applications · 2018Article
- Lasing in Live Mitotic and Non-Phagocytic Cells by Efficient Delivery of Microresonators.Scientific reports · 2017Article
- Deep Learning Automates the Quantitative Analysis of Individual Cells in Live-Cell Imaging Experiments.PLoS computational biology · 2016Article
- Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance.Nanomedicine (London, England) · 2016Article
- CellProfiler Tracer: exploring and validating high-throughput, time-lapse microscopy image data.BMC bioinformatics · 2015Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For phenotypic behavior to be understood in the context of cell lineage and local environment, properties of individual cells must be measured relative to population-wide traits. However, the inability to accurately identify, track and measure thousands of single cells via high-throughput microscopy has impeded dynamic studies of cell populations. We demonstrate unique labeling of cells, driven by the heterogeneous random uptake of fluorescent nanoparticles of different emission colors. By sequentially exposing a cell population to different particles, we generated a large number of unique digital codes, which corresponded to the cell-specific number of nanoparticle-loaded vesicles and were visible within a given fluorescence channel. When three colors are used, the assay can self-generate over 17,000 individual codes identifiable using a typical fluorescence microscope. The color-codes provided immediate visualization of cell identity and allowed us to track human cells with a success rate of 78% across image frames separated by 8 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.