ArticleNuclear medicine and biology2015
In vivo detection of hyperoxia-induced pulmonary endothelial cell death using (99m)Tc-duramycin.
Article in Nuclear medicine and biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Sex-specific differences in lung mitochondrial function and injury in rats exposed to hyperoxia.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Article
- Molecular Lung Imaging Following Exposure to Radiation Predicts Long-Term Survival in Rats.International journal of molecular sciences · 2026Article
- Lung uptake of two SPECT markers identifies sensitivity to hyperoxia-induced acute respiratory distress syndrome in rats.Frontiers in physiology · 2025Article
- Biomarkers to Predict Lethal Radiation Injury to the Rat Lung.International journal of molecular sciences · 2023Article
- In vivo molecular imaging stratifies rats with different susceptibilities to hyperoxic acute lung injury.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Mechanism of Action of Ribosomally Synthesized and Post-Translationally Modified Peptides.Chemical reviews · 2022Review
- Upregulating carnitine palmitoyltransferase 1 attenuates hyperoxia-induced endothelial cell dysfunction and persistent lung injury.Respiratory research · 2022Article
- Development of Duramycin-Based Molecular Probes for Cell Death Imaging.Molecular imaging and biology · 2022Review
- Fatty Acid Oxidation Protects against Hyperoxia-induced Endothelial Cell Apoptosis and Lung Injury in Neonatal Mice.American journal of respiratory cell and molecular biology · 2019Article
- Gadolinium-Based Nanoparticles for Theranostic MRI-Guided Radiosensitization in Hepatocellular Carcinoma.Frontiers in bioengineering and biotechnology · 2019Article
- Hyperoxia Causes Mitochondrial Fragmentation in Pulmonary Endothelial Cells by Increasing Expression of Pro-Fission Proteins.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- Hyperoxia induces paracellular leak and alters claudin expression by neonatal alveolar epithelial cells.Pediatric pulmonology · 2018Article
- Protection by Inhaled Hydrogen Therapy in a Rat Model of Acute Lung Injury can be Tracked in vivo Using Molecular Imaging.Shock (Augusta, Ga.) · 2017Article
- Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes.Chemical reviews · 2017Review
- 99MTc-Hexamethylpropyleneamine Oxime Imaging for Early Detection of Acute Lung Injury in Rats Exposed to Hyperoxia or Lipopolysaccharide Treatment.Shock (Augusta, Ga.) · 2016Article
- Biomarkers for Radiation Pneumonitis Using Noninvasive Molecular Imaging.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2016Article
- Imaging-based diagnosis of acute renal allograft rejection.World journal of transplantation · 2016Review
- Monitoring Apoptosis of Breast Cancer Xenograft After Paclitaxel Treatment With 99mTc-Labeled Duramycin SPECT/CT.Molecular imaging · 2016Article
- Characterization of [(99m)Tc]Duramycin as a SPECT Imaging Agent for Early Assessment of Tumor Apoptosis.Molecular imaging and biology · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
introduction(99m)Tc-duramycin, DU, is a SPECT biomarker of tissue injury identifying cell death. The objective of this study is to investigate the potential of DU imaging to quantify capillary endothelial cell death in rat lung injury resulting from hyperoxia exposure as a model of acute lung injury.
methodsRats were exposed to room air (normoxic) or >98% O2 for 48 or 60 hours. DU was injected i.v. in anesthetized rats, scintigraphy images were acquired at steady-state, and lung DU uptake was quantified from the images. Post-mortem, the lungs were removed for histological studies. Sequential lung sections were immunostained for caspase activation and endothelial and epithelial cells.
resultsLung DU uptake increased significantly (p<0.001) by 39% and 146% in 48-hr and 60-hr exposed rats, respectively, compared to normoxic rats. There was strong correlation (r(2)=0.82, p=0.005) between lung DU uptake and the number of cleaved caspase 3 (CC3) positive cells, and endothelial cells accounted for more than 50% of CC3 positive cells in the hyperoxic lungs. Histology revealed preserved lung morphology through 48 hours. By 60 hours there was evidence of edema, and modest neutrophilic infiltrate.
conclusionsRat lung DU uptake in vivo increased after just 48 hours of >98% O2 exposure, prior to the onset of any substantial evidence of lung injury. These results suggest that apoptotic endothelial cells are the primary contributors to the enhanced DU lung uptake, and support the utility of DU imaging for detecting early endothelial cell death in vivo.
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