ArticleThe Plant cell2014
Arabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Distinct features of ribonucleotides within genomic DNA in Aicardi-Goutières syndrome ortholog mutants ofiScience · 2024Article
- Mechanistic insights into DNA damage recognition and checkpoint control in plants.Nature plants · 2024Review
- DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis.Nature communications · 2023Article
- Distinct features of ribonucleotides within genomic DNA in Aicardi-Goutières syndrome (AGS)-ortholog mutants ofbioRxiv : the preprint server for biology · 2023Article
- Ribonucleotide and R-Loop Damage in Plastid DNA and Mitochondrial DNA during Maize Development.Plants (Basel, Switzerland) · 2023Article
- The ATR-WEE1 kinase module promotes SUPPRESSOR OF GAMMA RESPONSE 1 translation to activate replication stress responses.The Plant cell · 2023Article
- Regulation of Heat Stress inInternational journal of molecular sciences · 2022Article
- A Mutation in DNA Polymerase α RescuesInternational journal of molecular sciences · 2021Article
- G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability.Plant physiology · 2021Article
- Buying time: FASCIATA1 deficiency rescues wee1 plants from replication stress by delaying mitosis.Plant physiology · 2021Article
- The ATR-WEE1 kinase module inhibits the MAC complex to regulate replication stress response.Nucleic acids research · 2021Article
- A novel WEE1 pathway for replication stress responses.Nature plants · 2021Article
- Ribonucleotide incorporation into DNA during DNA replication and its consequences.Critical reviews in biochemistry and molecular biology · 2021Review
- Article
- Identification of the Association Between Toll-Like Receptors and T-Cell Activation in Takayasu's Arteritis.Frontiers in immunology · 2021Article
- OsMre11 Is Required for Mitosis during Rice Growth and Development.International journal of molecular sciences · 2020Article
- Evolutionary History and Activity of RNase H1-Like Proteins in Arabidopsis thaliana.Plant & cell physiology · 2020Article
- Cloning, transformation and expression of cell cycle-associated protein kinaseJournal, genetic engineering & biotechnology · 2018Article
- RAD51 and RTEL1 compensate telomere loss in the absence of telomerase.Nucleic acids research · 2018Article
- The Structure-Specific Endonucleases MUS81 and SEND1 Are Essential for Telomere Stability in Arabidopsis.The Plant cell · 2016Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The WEE1 kinase is an essential cell cycle checkpoint regulator in Arabidopsis thaliana plants experiencing replication defects. Whereas under non-stress conditions WEE1-deficient plants develop normally, they fail to adapt to replication inhibitory conditions, resulting in the accumulation of DNA damage and loss of cell division competence. We identified mutant alleles of the genes encoding subunits of the ribonuclease H2 (RNase H2) complex, known for its role in removing ribonucleotides from DNA-RNA duplexes, as suppressor mutants of WEE1 knockout plants. RNase H2 deficiency triggered an increase in homologous recombination (HR), correlated with the accumulation of γ-H2AX foci. However, as HR negatively impacts the growth of WEE1-deficient plants under replication stress, it cannot account for the rescue of the replication defects of the WEE1 knockout plants. Rather, the observed increase in ribonucleotide incorporation in DNA indicates that the substitution of deoxynucleotide with ribonucleotide abolishes the need for WEE1 under replication stress. Strikingly, increased ribonucleotide incorporation in DNA correlated with the occurrence of small base pair deletions, identifying the RNase H2 complex as an important suppressor of genome instability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.