ArticleEukaryotic cell2014
The contractile vacuole as a key regulator of cellular water flow in Chlamydomonas reinhardtii.
Article in Eukaryotic cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Phenotypic heterogeneity in a batch culture of Chlamydomonas reinhardtii with different light tolerances.PloS one · 2026Article
- Frog-killing chytrid fungus deploys different strategies to regulate intracellular pressure in developmental states that have or lack a cell wall.Current biology : CB · 2025Article
- Article
- Multi-omics analysis of green lineage osmotic stress pathways unveils crucial roles of different cellular compartments.Nature communications · 2024Article
- A conserved pressure-driven mechanism for regulating cytosolic osmolarity.Current biology : CB · 2023Article
- A conserved pressure-driven mechanism for regulating cytosolic osmolarity.bioRxiv : the preprint server for biology · 2023Article
- The old and the new about the contractile vacuole of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- Hyperosmotic stress-induced microtubule disassembly in Chlamydomonas reinhardtii.BMC plant biology · 2022Article
- Chlamydomonas reinhardtii cellular compartments and their contribution to intracellular calcium signalling.Journal of experimental botany · 2021Review
- Single-cell visualization and quantification of trace metals inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Potassium channel KCN11 is required for maintaining cellular osmolarity during nitrogen starvation to control proper cell physiology and TAG accumulation inBiotechnology for biofuels · 2020Article
- Cell wall integrity signaling regulates cell wall-related gene expression in Chlamydomonas reinhardtii.Scientific reports · 2019Article
- From molecular manipulation of domesticatedeLife · 2018Review
- Spatial and temporal specificity of CaThe New phytologist · 2016Article
- Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity.Journal of cell science · 2016Article
- Genome-wide expression profiles of Pyropia haitanensis in response to osmotic stress by using deep sequencing technology.BMC genomics · 2015Article
- An Atypical Aquaporin 1 Derived From Paramecium multimicronucleatum Functions as a Multifunctional Channel That Permeates Water, Glycerol, and Urea.The Journal of eukaryotic microbiologyArticle
- Resolving the homology-function relationship through comparative genomics of membrane-trafficking machinery and parasite cell biology.Molecular and biochemical parasitologyReview
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most freshwater flagellates use contractile vacuoles (CVs) to expel excess water. We have used Chlamydomonas reinhardtii as a green model system to investigate CV function during adaptation to osmotic changes in culture medium. We show that the contractile vacuole in Chlamydomonas is regulated in two different ways. The size of the contractile vacuoles increases during cell growth, with the contraction interval strongly depending on the osmotic strength of the medium. In contrast, there are only small fluctuations in cytosolic osmolarity and plasma membrane permeability. Modeling of the CV membrane permeability indicates that only a small osmotic gradient is necessary for water flux into the CV, which most likely is facilitated by the aquaporin major intrinsic protein 1 (MIP1). We show that MIP1 is localized to the contractile vacuole, and that the expression rate and protein level of MIP1 exhibit only minor fluctuations under different osmotic conditions. In contrast, SEC6, a protein of the exocyst complex that is required for the water expulsion step, and a dynamin-like protein are upregulated under strong hypotonic conditions. The overexpression of a CreMIP1-GFP construct did not change the physiology of the CV. The functional implications of these results are discussed.
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