ArticleApplied and environmental microbiology2014
Calcium-Enhanced Twitching Motility in Xylella fastidiosa Is Linked to a Single PilY1 Homolog.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- The calcium signal regulatory network and the roles of calcium-binding proteins in bacteria.World journal of microbiology & biotechnology · 2026Review
- The stoichiometry of minor-to-major pilins regulates the dynamic activity of the type IVa competence pilus in Vibrio cholerae.PLoS genetics · 2026Article
- PilY proteins: bimodular drivers of type IV pilus versatility.Trends in microbiology · 2026Review
- Diversity, classification, and evolution of myxobacterial PilY1 proteins.Frontiers in microbiology · 2026Article
- Mechanical forces and ligand binding modulateLife science alliance · 2025Article
- XadA-like adhesin XADA2 regulates biofilm formation in X. fastidiosa subsp. fastidiosa putatively by engaging oleic-acid derived oxylipins.Molecular biology reports · 2025Article
- Deciphering the enigmatic PilY1 ofBiochemistry and biophysics reports · 2024Article
- Calcium modulation of bacterial wilt disease on potato.Applied and environmental microbiology · 2024Article
- Comparative Genomics ofMicroorganisms · 2022Article
- A noncanonical cytochromeProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Calcium transcriptionally regulates movement, recombination and other functions of Xylella fastidiosa under constant flow inside microfluidic chambers.Microbial biotechnology · 2020Article
- Effects of Calcium and Signal Sensing Systems onFrontiers in microbiology · 2020Article
- Ionomic Differences between Susceptible and Resistant Olive Cultivars Infected byPathogens (Basel, Switzerland) · 2019Article
- Genomic Diversity and Recombination among Xylella fastidiosa Subspecies.Applied and environmental microbiology · 2019Article
- A NaMolecular plant pathology · 2019Article
- Enhanced calcium carbonate-biofilm complex formation by alkali-generating Lysinibacillus boronitolerans YS11 and alkaliphilic Bacillus sp. AK13.AMB Express · 2019Article
- A Short Protocol for Gene Knockout and Complementation in Xylella fastidiosa Shows that One of the Type IV Pilin Paralogs (PD1926) Is Needed for Twitching while Another (PD1924) Affects Pilus Number and Location.Applied and environmental microbiology · 2018Article
- Xylella fastidiosa: an examination of a re-emerging plant pathogen.Molecular plant pathology · 2018Article
- Assessing Travel Conditions: Environmental and Host Influences On Bacterial Surface Motility.Journal of bacteriology · 2018Article
- Natural Competence of Xylella fastidiosa Occurs at a High Frequency Inside Microfluidic Chambers Mimicking the Bacterium's Natural Habitats.Applied and environmental microbiology · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant-pathogenic bacterium Xylella fastidiosa is restricted to the xylem vessel environment, where mineral nutrients are transported through the plant host; therefore, changes in the concentrations of these elements likely impact the growth and virulence of this bacterium. Twitching motility, dependent on type IV pili (TFP), is required for movement against the transpiration stream that results in basipetal colonization. We previously demonstrated that calcium (Ca) increases the motility of X. fastidiosa, although the mechanism was unknown. PilY1 is a TFP structural protein recently shown to bind Ca and to regulate twitching and adhesion in bacterial pathogens of humans. Sequence analysis identified three pilY1 homologs in X. fastidiosa (PD0023, PD0502, and PD1611), one of which (PD1611) contains a Ca-binding motif. Separate deletions of PD0023 and PD1611 resulted in mutants that still showed twitching motility and were not impaired in attachment or biofilm formation. However, the response of increased twitching at higher Ca concentrations was lost in the pilY1-1611 mutant. Ca does not modulate the expression of any of the X. fastidiosa PilY1 homologs, although it increases the expression of the retraction ATPase pilT during active movement. The evidence presented here suggests functional differences between the PilY1 homologs, which may provide X. fastidiosa with an adaptive advantage in environments with high Ca concentrations, such as xylem sap.
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