ArticlePloS one2014
Effects of negative pressure wound therapy on mesenchymal stem cells proliferation and osteogenic differentiation in a fibrin matrix.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Effect of Topical Administration of Fractions and Isolated Molecules from Plant Extracts on Skin Wound Healing: A Systematic Review of Murine Experimental Models.Mediators of inflammation · 2016Pooled it
- Influence of Unidirectional Vacuum Application on Bone Healing in Maxillofacial Surgery.Cells · 2025Article
- Continuous negative-pressure wound therapy improves the survival rate of skin grafts and shortens the time required for skin graft survival.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Article
- The role of integrin family in bone metabolism and tumor bone metastasis.Cell death discovery · 2023Review
- Negative-Pressure Wound Therapy: What We Know and What We Need to Know.Advances in experimental medicine and biology · 2023Article
- Combined computational analysis and cytology show limited depth osteogenic effect on bone defects in negative pressure wound therapy.Frontiers in bioengineering and biotechnology · 2023Article
- Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis.Autophagy · 2022Article
- A bioreactor for studying negative pressure wound therapy on skin grafts.International wound journal · 2022Article
- Negative pressure wound therapy enhances bone regeneration compared with conventional therapy in a rabbit radius gap-healing model.Experimental and therapeutic medicine · 2021Article
- Potential molecular mechanisms of negative pressure in promoting wound healing.International wound journal · 2020Article
- A case series of negative pressure wound therapy as a promising treatment in patients with burn injury.International journal of surgery case reports · 2020Article
- Negative Pressure Therapy in the Regeneration of the Sciatic Nerve Using Vacuum - Assisted Closure in a Rabbit Model.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Negative pressure wound therapy promotes muscle-derived stem cell osteogenic differentiation through MAPK pathway.Journal of cellular and molecular medicine · 2018Article
- [Effects of long time different negative pressures on osteogenic differentiation of rabbit bone mesenchymal stem cells].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2017Article
- Enhancement of Bone-Marrow-Derived Mesenchymal Stem Cell Angiogenic Capacity by NPWT for a Combinatorial Therapy to Promote Wound Healing with Large Defect.BioMed research international · 2017Article
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Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vacuum-assisted closure (VAC) negative pressure wound therapy (NPWT) has been proven to be an effective therapeutic method for the treatment of recalcitrant wounds. However, its role in bone healing remains to be unclear. Here, we investigated the effects of NPWT on rat periosteum-derived mesenchymal stem cells (P-MSCs) proliferation and osteoblastic differentiation in a 3D fibrin matrix. P-MSCs underwent primary culture for three passages before being used to construct cell clots. The fibrin clots were incubated with NPWT under continuous suction at -125 mmHg in a subatmospheric perfusion bioreactor. Clots exposed to atmospheric pressure served as the static control. Compared to the control group, cell proliferation significantly increased in NPWT group after incubation for 3 days. There was no statistical difference in apoptosis rate between two groups. The ALP activity and mineralization of P-MSCs all increased under continuous suction. The expressions of collagen type 1 and transcription factor Cbfa-1 were higher at the 1-, 3-, and 7-day timepoints and the expressions of osteocalcin and integrin β5 were higher at the 3-, and 7-day timepoints in the NPWT group. These results indicate that a short time treatment with NPWT, applied with continuous suction at -125 mmHg, can enhance cellular proliferation of P-MSCs and induce the differentiation toward an osteogenic phenotype. The mechanotransduction molecule integrin β5 was found to be highly expressed after NPWT treatment, which indicates that NPWT may play a positive role in fracture healing through enhance bone formation and decrease bone resorption.
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