ArticleACS synthetic biology2015
Synthetic biology toolbox for controlling gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
Article in ACS synthetic biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 216 citations in OpenAlex.
- Photosynthetic production of glutamine through metabolic analysis-based engineering ofMetabolic engineering communications · 2026Article
- ModifiedMicroorganisms · 2026Article
- A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.Applied and environmental microbiology · 2026Article
- A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.bioRxiv : the preprint server for biology · 2026Article
- CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.BMC biotechnology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- High enzyme promiscuity in lignin degradation mechanisms inApplied and environmental microbiology · 2025Article
- Engineering of the fast-growing cyanobacterium Synechococcus sp. PCC 11901 to synthesize astaxanthin.Biotechnology for biofuels and bioproducts · 2025Article
- Toward sustainable phycocyanin production using halo-alkaliphilic cyanobacteria: from direct air capture of carbon dioxide to biorefinery.Frontiers in microbiology · 2025Review
- Biotechnological Applications of Cyanobacteria: Synechocystis and Synechococcus Strains.Advances in biochemical engineering/biotechnology · 2025Review
- Development of a xylose-inducible and glucose-insensitive expression system for Parageobacillus thermoglucosidasius.Applied microbiology and biotechnology · 2024Article
- Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics: A comprehensive review.Environmental science and ecotechnology · 2024Review
- Species-specific design of artificial promoters by transfer-learning based generative deep-learning model.Nucleic acids research · 2024Article
- The Impact of Second-Shell Nucleotides on Ligand Specificity in Cyclic Dinucleotide Riboswitches.Biochemistry · 2024Article
- Toolbox of Characterized Genetic Parts forACS synthetic biology · 2024Article
- Optogenetic control of gene expression in the cyanobacteriumFrontiers in bioengineering and biotechnology · 2024Article
- Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.ACS synthetic biology · 2023Article
- The current situations and limitations of genetic engineering in cyanobacteria: a mini review.Molecular biology reports · 2023Review
- Identification of a Far-Red Light-Inducible Promoter that Exhibits Light Intensity Dependency and Reversibility in a Cyanobacterium.ACS synthetic biology · 2023Article
- Recent developments in the production and utilization of photosynthetic microorganisms for food applications.Heliyon · 2023Review
24 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The application of synthetic biology requires characterized tools to precisely control gene expression. This toolbox of genetic parts previously did not exist for the industrially promising cyanobacterium, Synechococcus sp. strain PCC 7002. To address this gap, two orthogonal constitutive promoter libraries, one based on a cyanobacterial promoter and the other ported from Escherichia coli, were built and tested in PCC 7002. The libraries demonstrated 3 and 2.5 log dynamic ranges, respectively, but correlated poorly with E. coli expression levels. These promoter libraries were then combined to create and optimize a series of IPTG inducible cassettes. The resultant induction system had a 48-fold dynamic range and was shown to out-perform Ptrc constructs. Finally, a RBS library was designed and tested in PCC 7002. The presented synthetic biology toolbox will enable accelerated engineering of PCC 7002.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.