ArticlePloS one2014
The effects of controlled release of neurotrophin-3 from PCLA scaffolds on the survival and neuronal differentiation of transplanted neural stem cells in a rat spinal cord injury model.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Combined application of neural stem/progenitor cells and scaffolds on locomotion recovery following spinal cord injury in rodents: a systematic review and meta-analysis.Neurosurgical review · 2022Pooled it
- Reprogramming the Inflammatory Response to Promote Neural Stem Cell Function After Spinal Cord Injury.Molecular neurobiology · 2026Review
- Neurotrophin-3 (NT-3) as a Potential Biomarker of the Peripheral Nervous System Damage Following Breast Cancer Treatment.Pathophysiology : the official journal of the International Society for Pathophysiology · 2023Article
- Current Concepts of Biomaterial Scaffolds and Regenerative Therapy for Spinal Cord Injury.International journal of molecular sciences · 2023Review
- Prospects for the use of olfactory mucosa cells in bioprinting for the treatment of spinal cord injuries.World journal of clinical cases · 2023Review
- Silk fibroin carriers with sustained release capacity for treating neurological diseases.Frontiers in pharmacology · 2023Review
- Integrated printed BDNF/collagen/chitosan scaffolds with low temperature extrusion 3D printer accelerated neural regeneration after spinal cord injury.Regenerative biomaterials · 2021Article
- Human-Induced Neural and Mesenchymal Stem Cell Therapy Combined with a Curcumin Nanoconjugate as a Spinal Cord Injury Treatment.International journal of molecular sciences · 2021Article
- Injectable, macroporous scaffolds for delivery of therapeutic genes to the injured spinal cord.APL bioengineering · 2021Article
- Therapeutic Plasticity of Neural Stem Cells.Frontiers in neurology · 2020Review
- Regenerative Therapies for Spinal Cord Injury.Tissue engineering. Part B, Reviews · 2019Review
- Matrices, scaffolds & carriers for cell delivery in nerve regeneration.Experimental neurology · 2019Review
- Transplantation of Schwann Cells Inside PVDF-TrFE Conduits to Bridge Transected Rat Spinal Cord Stumps to Promote Axon Regeneration Across the Gap.Journal of visualized experiments : JoVE · 2017Article
- Stem cells for spinal cord injury: Strategies to inform differentiation and transplantation.Biotechnology and bioengineering · 2017Review
- Resveratrol pretreatment attenuates injury and promotes proliferation of neural stem cells following oxygen-glucose deprivation/reoxygenation by upregulating the expression of Nrf2, HO-1 and NQO1 in vitro.Molecular medicine reports · 2016Article
- Neurotrophic Factors and Their Potential Applications in Tissue Regeneration.Archivum immunologiae et therapiae experimentalis · 2016Review
- Lentivirus-mediated Persephin over-expression in Parkinson's disease rats.Neural regeneration research · 2015Article
- Article
- Stem Cell Therapy for Spinal Cord Injury.Cell transplantationArticle
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neural stem cells (NSCs) have emerged as a potential source for cell replacement therapy following spinal cord injury (SCI). However, poor survival and low neuronal differentiation remain major obstacles to the use of NSCs. Biomaterials with neurotrophic factors are promising strategies for promoting the proliferation and differentiation of NSCs. Silk fibroin (SF) matrices were demonstrated to successfully deliver growth factors and preserve their potency. In this study, by incorporating NT-3 into a SF coating, we successfully developed NT-3-immobilized scaffolds (membranes and conduits). Sustained release of bioactive NT-3 from the conduits for up to 8 weeks was achieved. Cell viability was confirmed using live/dead staining after 14 days in culture. The efficacy of the immobilized NT-3 was confirmed by assessing NSC neuronal differentiation in vitro. NSC neuronal differentiation was 55.2 ± 4.1% on the NT-3-immobilized membranes, which was significantly higher than that on the NT-3 free membrane. Furthermore, 8 weeks after the NSCs were seeded into conduits and implanted in rats with a transected SCI, the conduit+NT-3+NSCs group achieved higher NSC survival (75.8 ± 15.1%) and neuronal differentiation (21.5 ± 5.2%) compared with the conduit+NSCs group. The animals that received the conduit+NT-3+NSCs treatment also showed improved functional outcomes, as well as increased axonal regeneration. These results indicate the feasibility of fabricating NT-3-immobilized scaffolds using the adsorption of NT-3/SF coating method, as well as the potential of these scaffolds to induce SCI repair by promoting survival and neuronal differentiation of transplanted NSCs.
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