ArticlePloS one2014
Identification of protein biomarkers for cervical cancer using human cervicovaginal fluid.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Uncovering candidate protein(s) in murine vaginal lavage fluid linked to sperm-agglutinating Escherichia coli-induced infertility menace: a discovery phase analysis.Archives of microbiology · 2026Article
- Aptamer-Based Graphene Field-Effect Transistor Biosensor for Cytokine Detection in Undiluted Physiological Media for Cervical Carcinoma Diagnosis.Biosensors · 2025Article
- Unveiling diagnostic and therapeutic strategies for cervical cancer: biomarker discovery through proteomics approaches and exploring the role of cervical cancer stem cells.Frontiers in oncology · 2023Review
- Applications of MALDI-MS/MS-Based Proteomics in Biomedical Research.Molecules (Basel, Switzerland) · 2022Review
- Review
- Point-of-care diagnosis of cervical cancer: potential protein biomarkers in cervicovaginal fluid.Turkish journal of biology = Turk biyoloji dergisi · 2022Review
- New Trends in the Detection of Gynecological Precancerous Lesions and Early-Stage Cancers.Cancers · 2021Review
- Human disease biomarker panels through systems biology.Biophysical reviews · 2021Review
- Review
- Article
- Quantitative proteomic profiling of Cervicovaginal fluid from pregnant women with term and preterm birth.Proteome science · 2021Article
- miRNA profiling of primate cervicovaginal lavage and extracellular vesicles reveals miR-186-5p as a potential antiretroviral factor in macrophages.FEBS open bio · 2020Article
- Combined Detection of ACTN4 and SCC-Ag is a Promising Serological Biomarker for Cervical Intraepithelial Neoplasia 3 or Worse: A Case-Control Study.Risk management and healthcare policy · 2020Article
- Metabolomic biomarkers in cervicovaginal fluid for detecting endometrial cancer through nuclear magnetic resonance spectroscopy.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- Cervico-vaginal mucus (CVM) - an accessible source of immunologically informative biomolecules.Veterinary research communications · 2018Article
- Improved detection of biomarkers in cervico-vaginal mucus (CVM) from postpartum cattle.BMC veterinary research · 2018Article
- Human papilloma virus: Apprehending the link with carcinogenesis and unveiling new research avenues (Review).International journal of oncology · 2018Review
- Candidate biomarkers in the cervical vaginal fluid for the (self-)diagnosis of cervical precancer.Archives of gynecology and obstetrics · 2018Review
- Molecular Signatures of High-Grade Cervical Lesions.Frontiers in oncology · 2018Article
- Liquid biopsy for pediatric central nervous system tumors.NPJ precision oncology · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesCervicovaginal fluid (CVF) can be considered as a potential source of biomarkers for diseases of the lower female reproductive tract. The fluid can easily be collected, thereby offering new opportunities such as the development of self tests. Our objective was to identify a CVF protein biomarker for cervical cancer or its precancerous state.
methodsA differential proteomics study was set up using CVF samples from healthy and precancerous women. Label-free spectral counting was applied to quantify protein abundances.
resultsThe proteome analysis revealed 16 candidate biomarkers of which alpha-actinin-4 (p = 0.001) and pyruvate kinase isozyme M1/M2 (p = 0.014) were most promising. Verification of alpha-actinin-4 by ELISA (n = 28) showed that this candidate biomarker discriminated between samples from healthy and both low-risk and high-risk HPV-infected women (p = 0.009). Additional analysis of longitudinal samples (n = 29) showed that alpha-actinin-4 levels correlated with virus persistence and clearing, with a discrimination of approximately 18 pg/ml.
conclusionsOur results show that CVF is an excellent source of protein biomarkers for detection of lower female genital tract pathologies and that alpha-actinin-4 derived from CVF is a promising candidate biomarker for the precancerous state of cervical cancer. Further studies regarding sensitivity and specificity of this biomarker will demonstrate its utility for improving current screening programs and/or its use for a cervical cancer self-diagnosis test.
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