ArticleBioconjugate chemistry2014
Universal antibody conjugation to nanoparticles using the Fcγ receptor I (FcγRI): quantitative profiling of membrane biomarkers.
Article in Bioconjugate chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Bispecific antibody targeting of lipid nanoparticles.bioRxiv : the preprint server for biology · 2024Article
- A Simple and Versatile Strategy for Oriented Immobilization of His-Tagged Proteins on Magnetic Nanoparticles.Bioconjugate chemistry · 2023Article
- Article
- Highly sensitive photoelectrochemical immunosensor for detecting cancer marker CA19-9 based on a new SnSe quantum dot.Mikrochimica acta · 2023Article
- Antibody-conjugated nanoparticles for target-specific drug delivery of chemotherapeutics.Beilstein journal of nanotechnology · 2023Review
- Characterization of FcγRIa (CD64) as a Ligand Molecule for Site-Specific IgG1 Capture: A Side-By-Side Comparison with Protein A.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Versatile and Robust Method for Antibody Conjugation to Nanoparticles with High Targeting Efficiency.Pharmaceutics · 2021Article
- Impact of the conjugation of antibodies to the surfaces of polymer nanoparticles on the immune cell targeting abilities.Nano convergence · 2021Article
- Antibody Self-Assembly Maximizes Cytoplasmic Immunostaining Accuracy of Compact Quantum Dots.Chemistry of materials : a publication of the American Chemical Society · 2021Article
- Metal Nanoparticles/MoSACS applied materials & interfaces · 2021Article
- Point of Care Test Technology Suitable for Early Detection and Monitoring of Ischemic Stroke.Current neurovascular research · 2021Article
- Impact of conjugation strategies for targeting of antibodies in gold nanoparticles for ultrasensitive detection of 17β-estradiol.Scientific reports · 2019Article
- Quantum Dot Surface Engineering: Toward Inert Fluorophores with Compact Size and Bright, Stable Emission.Coordination chemistry reviews · 2016Article
- Highly sensitive detection of protein biomarkers via nuclear magnetic resonance biosensor with magnetically engineered nanoferrite particles.International journal of nanomedicineArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Antibodies are a class of molecules widely used in bioengineering and nanomedicine for applications involving protein recognition and targeting. Here we report an efficient method for universal conjugation of antibodies to lipid-coated nanoparticles using radially oriented FcγRIs. This method is performed in physiological solution with no additional coupling reagents, thereby avoiding problems with antibody stability and functionality. Coupling to the Fc region of the antibody avoids aggregation and polymerization allowing high yield. In addition, the antibody is oriented perpendicular to the surface so that the binding sites are fully functional. Using this method we demonstrate quantitative profiling of a panel of four membrane-bound cancer biomarkers (claudin-4, mesothelin, mucin-4, and cadherin-11) on four cell lines (Panc-1, MIA PaCa-2, Capan-1, and HPDE). We show that by designing the lipid coating to minimize aggregation and nonspecific binding, we can obtain absolute values of biomarker expression levels as number per unit area on the cell surface. This method is applicable to a wide range of technologies, including solution based protein detection assays and active targeting of cell surface membrane biomarkers.
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Registered trials
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