Evidence map›Paper›PMID 25215182›Full record

ArticleAvicenna journal of medical biotechnology2014

Genetic Profile Variation in Vaccine Strains and Clinical Isolates of Bordetella pertussis Recovered from Iranian Patients.

Faezeh Haghighi, Fereshteh Shahcheraghi, Ebrahim Abbasi, Seyed Saeed Eshraghi, Hojjat Zeraati, Seyed Ali Javad Mousavi, Hossein Asgarian-Omran, Masoumeh Douraghi, Fazel Shokri

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In one paragraph

Article in Avicenna journal of medical biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Characterization ofPathogens (Basel, Switzerland) · 2022
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Strain variation and antigenic divergence among Bordetella pertussis circulating strains isolated from patients in Iran.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018
    Article
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Faezeh HaghighiDivision of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Fereshteh ShahcheraghiDepartment of Bacteriology and Microbiology, Pasteur Institute of Iran, Tehran, Iran.
Ebrahim AbbasiDepartment of Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran.
Seyed Saeed EshraghiDivision of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Hojjat ZeraatiDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Seyed Ali Javad MousaviDepartment of Pulmonology, Hazrat Rasool Hospital, Iran University of Medical Sciences, Tehran, Iran.
Hossein Asgarian-OmranDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran ; Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Masoumeh DouraghiDivision of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Fazel ShokriDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran ; Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundRe-emergence of pertussis has been reported in Iran despite a high rate of vaccination coverage. Low efficacy of the vaccine might be due to the genetic divergence between clinical versus vaccine strains. In the current study, the genetic profiles of clinical isolates and vaccine strains of Bordetella pertussis (B. pertussis) were assessed by using Pulsed Field Gel Electrophoresis (PFGE).

methodsFollowing phenotypic and molecular identification of isolates, XbaI-digested genomic DNA of 5 clinical isolates, 2 vaccine strains and a Tohama I strain were analyzed by PFGE along with B. parapertussis as a control.

resultsSeven distinct PFGE profiles were found among all examined isolates/strains. In 5 clinical isolates, 4 profiles were identified whereas the vaccine strains displayed 2 distinct profiles. The reference strain, Tohama I had a distinct profile. Vaccine and clinical profiles had low similarity, with relatedness of approximately 40%.

conclusionThe genetic profiles of B. pertussis were different between circulating isolates and vaccine strains used in the national vaccination programs. Since new genetic profiles of B. pertussis can be disseminated periodically, the profiles of isolates circulating in the population should be monitored over the course of the re-emergence.

Indexed as

Bordetella pertussisPFGE profileVaccinationWhooping cough

Identifiers

PMID25215182
PMCPMC4147105

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.