ArticlePlant physiology2014
Peroxisomal ubiquitin-protein ligases peroxin2 and peroxin10 have distinct but synergistic roles in matrix protein import and peroxin5 retrotranslocation in Arabidopsis.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Building peroxisomes: perspectives on plant peroxins.Biochemical Society transactions · 2026Review
- Wood-specific modification of glucuronoxylan can enhance growth in Populus.Journal of experimental botany · 2026Article
- Identifying and characterizing a missing peroxin-PEX8-in Arabidopsis thaliana.The Plant cell · 2025Article
- Biochemical and Transcriptomic Analysis Reveals Low Temperature-Driven Oxidative Stress in PupalInsects · 2025Article
- Fluorescent fatty acid conjugates for live cell imaging of peroxisomes.Nature communications · 2024Article
- Plant peroxisome proteostasis-establishing, renovating, and dismantling the peroxisomal proteome.Essays in biochemistry · 2022Review
- Ubiquitin-conjugating activity by PEX4 is required for efficient protein transport to peroxisomes in Arabidopsis thaliana.The Journal of biological chemistry · 2022Article
- Image-Based Analysis Revealing the Molecular Mechanism of Peroxisome Dynamics in Plants.Frontiers in cell and developmental biology · 2022Review
- The Structure of theFrontiers in cell and developmental biology · 2022Article
- ATG8-Interacting Motif: Evolution and Function in Selective Autophagy of Targeting Biological Processes.Frontiers in plant science · 2021Review
- Multilevel Regulation of Peroxisomal Proteome by Post-Translational Modifications.International journal of molecular sciences · 2019Review
- PEX16 contributions to peroxisome import and metabolism revealed by viable Arabidopsis pex16 mutants.Journal of integrative plant biology · 2019Article
- APlant direct · 2019Article
- Dynamics of Peroxisome Homeostasis and Its Role in Stress Response and Signaling in Plants.Frontiers in plant science · 2019Review
- AProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Repertoire of plant RING E3 ubiquitin ligases revisited: New groups counting gene families and single genes.PloS one · 2018Article
- Peroxisome Function, Biogenesis, and Dynamics in Plants.Plant physiology · 2018Review
- Disparate peroxisome-related defects in Arabidopsis pex6 and pex26 mutants link peroxisomal retrotranslocation and oil body utilization.The Plant journal : for cell and molecular biology · 2017Article
- The PEX1 ATPase Stabilizes PEX6 and Plays Essential Roles in Peroxisome Biology.Plant physiology · 2017Article
- Plant peroxisomes: recent discoveries in functional complexity, organelle homeostasis, and morphological dynamics.Current opinion in plant biology · 2016Review
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3 authors at 1 institution in 1 country.
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Abstract
Peroxisomal matrix proteins carry peroxisomal targeting signals (PTSs), PTS1 or PTS2, and are imported into the organelle with the assistance of peroxin (PEX) proteins. From a microscopy-based screen to identify Arabidopsis (Arabidopsis thaliana) mutants defective in matrix protein degradation, we isolated unique mutations in PEX2 and PEX10, which encode ubiquitin-protein ligases anchored in the peroxisomal membrane. In yeast (Saccharomyces cerevisiae), PEX2, PEX10, and a third ligase, PEX12, ubiquitinate a peroxisome matrix protein receptor, PEX5, allowing the PEX1 and PEX6 ATP-hydrolyzing enzymes to retrotranslocate PEX5 out of the membrane after cargo delivery. We found that the pex2-1 and pex10-2 Arabidopsis mutants exhibited defects in peroxisomal physiology and matrix protein import. Moreover, the pex2-1 pex10-2 double mutant exhibited severely impaired growth and synergistic physiological defects, suggesting that PEX2 and PEX10 function cooperatively in the wild type. The pex2-1 lesion restored the unusually low PEX5 levels in the pex6-1 mutant, implicating PEX2 in PEX5 degradation when retrotranslocation is impaired. PEX5 overexpression altered pex10-2 but not pex2-1 defects, suggesting that PEX10 facilitates PEX5 retrotranslocation from the peroxisomal membrane. Although the pex2-1 pex10-2 double mutant displayed severe import defects of both PTS1 and PTS2 proteins into peroxisomes, both pex2-1 and pex10-2 single mutants exhibited clear import defects of PTS1 proteins but apparently normal PTS2 import. A similar PTS1-specific pattern was observed in the pex4-1 ubiquitin-conjugating enzyme mutant. Our results indicate that Arabidopsis PEX2 and PEX10 cooperate to support import of matrix proteins into plant peroxisomes and suggest that some PTS2 import can still occur when PEX5 retrotranslocation is slowed.
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