Evidence map›Paper›PMID 25214397›Full record

ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2014

Chronic alcohol exposure inhibits biotin uptake by pancreatic acinar cells: possible involvement of epigenetic mechanisms.

Padmanabhan Srinivasan, Rubina Kapadia, Arundhati Biswas, Hamid M Said

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Gastrointestinal and liver physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Biotin Supplementation Ameliorates Murine Colitis by Preventing NF-κB Activation.Cellular and molecular gastroenterology and hepatology · 2020
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Padmanabhan SrinivasanDepartment of Medical Research, Veterans Affairs Medical Center, Long Beach, California; and Departments of Medicine and Physiology/Biophysics, University of California, Irvine, California.
Rubina KapadiaDepartment of Medical Research, Veterans Affairs Medical Center, Long Beach, California; and Departments of Medicine and Physiology/Biophysics, University of California, Irvine, California.
Arundhati BiswasDepartment of Medical Research, Veterans Affairs Medical Center, Long Beach, California; and Departments of Medicine and Physiology/Biophysics, University of California, Irvine, California.
Hamid M SaidDepartment of Medical Research, Veterans Affairs Medical Center, Long Beach, California; and Departments of Medicine and Physiology/Biophysics, University of California, Irvine, California hmsaid@uci.edu.
University of California, Irvine · US

Funding

INTESTINAL VITAMIN H UPTAKE--MOLECULAR/CELL REGULATIONR01DK058057 · NIDDK · UNIVERSITY OF CALIFORNIA-IRVINE · PI SAID, HAMID M · 2000 to 2019
$6.8M
Physiology/Pathophysiology of Vitamin B1 Transport in Pancreatic Acinar CellsR01AA018071 · NIAAA · UNIVERSITY OF CALIFORNIA-IRVINE · PI SAID, HAMID M · 2009 to 2023
$6.3M
Mechanism/Regulation of Intestinal Thiamin UptakeR37DK056061 · NIDDK · UNIVERSITY OF CALIFORNIA-IRVINE · PI SAID, HAMID M · 2009 to 2018
$3.6M
NIAAA NIH HHS AA018071NIAAA NIH HHS R01 AA018071NIDDK NIH HHS DK58057NIDDK NIH HHS R37 DK056061
6 · The paper itself

Abstract

Chronic exposure to alcohol affects different physiological aspects of pancreatic acinar cells (PAC), but its effect on the uptake process of biotin is not known. We addressed this issue using mouse-derived pancreatic acinar 266-6 cells chronically exposed to alcohol and wild-type and transgenic mice (carrying the human SLC5A6 5'-promoter) fed alcohol chronically. First we established that biotin uptake by PAC is Na(+) dependent and carrier mediated and involves sodium-dependent multivitamin transporter (SMVT). Chronic exposure of 266-6 cells to alcohol led to a significant inhibition in biotin uptake, expression of SMVT protein, and mRNA as well as in the activity of the SLC5A6 promoter. Similarly, chronic alcohol feeding of wild-type and transgenic mice carrying the SLC5A6 promoter led to a significant inhibition in biotin uptake by PAC, as well as in the expression of SMVT protein and mRNA and the activity of the SLC5A6 promoters expressed in the transgenic mice. We also found that chronic alcohol feeding of mice is associated with a significant increase in the methylation status of CpG islands predicted to be in the mouse Slc5a6 promoters and a decrease in the level of expression of transcription factor KLF-4, which plays an important role in regulating SLC5A6 promoter activity. These results demonstrate, for the first time, that chronic alcohol exposure negatively impacts biotin uptake in PAC and that this effect is exerted (at least in part) at the level of transcription of the SLC5A6 gene and may involve epigenetic/molecular mechanisms.

Indexed as

Epigenesis, GeneticAcinar CellsAlcoholismAnimalsBiological Transport, ActiveBiotinCells, CulturedCpG IslandsDNA MethylationEthanolHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMicePancreasPromoter Regions, GeneticBiotinbiotin transporterEthanolKLF4 protein, humanKlf4 protein, mouseKruppel-Like Factor 4Kruppel-Like Transcription FactorsRNA, MessengerSodiumSymportersalcoholbiotinpancreatic acinar cellstransport

Identifiers

PMID25214397
PMCPMC4250263
OpenAlexW2156488107

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.