Evidence map›Paper›PMID 25213663›Full record

ArticleCancer gene therapy2014

Therapeutic adenoviral gene transfer of a glycosyltransferase for prevention of peritoneal dissemination and metastasis of gastric cancer.

Y I Kawamura, Y Adachi, D T Curiel, R Kawashima, R Kannagi, N Nishimoto, T Dohi

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Cancer gene therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 22 citations in OpenAlex.

  1. Review
  2. Review
  3. The story of the SdGlycoconjugate journal · 2023
    Review
  4. A roadmap for translational cancer glycoimmunology at single cell resolution.Journal of experimental & clinical cancer research : CR · 2022
    Review
  5. Review
  6. B4GALNT2 Controls SdChembiochem : a European journal of chemical biology · 2021
    Review
  7. Review
  8. Article
  9. The SdInternational journal of molecular sciences · 2020
    Article
  10. Article
  11. Article
  12. Issues on peritoneal metastasis of gastric cancer: an update.World journal of surgical oncology · 2019
    Review
  13. Article
  14. Review
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Y I Kawamura1] Department of Gastroenterology, Research Center for Hepatitis and Immunology, Research Institute, National Center for Global Health and Medicine, Chiba, Japan [2] CREST, Japan Science and Technology Agency, Tokyo, Japan.
Y AdachiLaboratory of Immune Regulation, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
D T CurielDivision of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Surgery, Pathology, and the Gene Therapy Center at UAB, University of Alabama at Birmingham, Birmingham, AL, USA.
R Kawashima1] Department of Gastroenterology, Research Center for Hepatitis and Immunology, Research Institute, National Center for Global Health and Medicine, Chiba, Japan [2] Department of Biochemistry, School of Allied Health Sciences, Kitasato University Graduate School of Medical Sciences, Kanagawa, Japan.
R Kannagi1] CREST, Japan Science and Technology Agency, Tokyo, Japan [2] Program of Molecular Pathology, Aichi Cancer Center, Nagoya, Japan.
N NishimotoLaboratory of Immune Regulation, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.
T Dohi1] Department of Gastroenterology, Research Center for Hepatitis and Immunology, Research Institute, National Center for Global Health and Medicine, Chiba, Japan [2] CREST, Japan Science and Technology Agency, Tokyo, Japan.
Osaka University · JPKitasato University · JPUniversity of Alabama at Birmingham · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Increased expression of sialyl Lewis(x/a) carbohydrates, ligands for E-selectin, correlates with clinically advanced stages and metastasis of gastric and colon cancers. In contrast, Sd(a) carbohydrate is abundantly detected in the normal gastrointestinal mucosa but dramatically reduced or lost in cancer tissues. A glycosyltransferase, β1,4N-acetylgalactosaminyltransferase 2 (B4GALNT2) that catalyzes Sd(a) carbohydrate synthesis, is silenced in cancer. In the present study, we aimed at reducing the expression of sialyl Lewis(x/a) of cancer cells in vivo by forced expression of B4GALNT2 and Sd(a), thereby preventing dissemination/metastasis, especially metastasis triggered by surgical maneuvers. We used a fiber-modified adenovirus (Ad) vector that contained a chimeric construct with a serotype 5 shaft and a serotype 3 knob. Using this Ad5/3 vector, we successfully introduced the B4GALNT2 gene into a human gastric cancer cell line KATO III in vitro and confirmed replacement of sialyl Lewis(x) to Sd(a) with a decrease in E-selectin-dependent adhesion. Administration of Ad5/3-B4GALNT2 vectors into the peritoneal cavity of mice after inoculation of KATO III cells with laparotomy significantly reduced the incidence of metastasis. Our results indicate that the transfer of a single gene encoding B4GALNT2 modified carbohydrate chains of cancer cells in vivo and decreased tumor dissemination and metastasis.

Indexed as

Gene Transfer TechniquesAdenoviridaeAnimalsCapsid ProteinsCell AdhesionCell Line, TumorDisease Models, AnimalEnzyme ActivationE-SelectinGene ExpressionGenetic VectorsGlycosyltransferasesHumansMaleMiceNeoplasm MetastasisCapsid ProteinsE-SelectinGlycosyltransferaseshexon capsid protein, Adenovirus

Identifiers

PMID25213663
OpenAlexW1995778171

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.