ArticleDevelopment, growth & differentiation2014
CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis.
Article in Development, growth & differentiation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 82 citations in OpenAlex.
- Efficient Microinjection and CRISPR/Cas9 Mediated Genome Editing in Urechis unicinctus.Marine biotechnology (New York, N.Y.) · 2026Article
- Neural signaling contributes to heart formation and growth in the invertebrate chordate, Ciona robusta.PLoS biology · 2026Article
- Engineering delivery platforms for CRISPR-Cas and their applications in healthcare, agriculture and beyond.Nanoscale advances · 2026Review
- Development of cell labeling and gene editing tools in urochordateMarine life science & technology · 2025Article
- A simple inland culture system provides insights into ascidian post-embryonic developmental physiology.Open biology · 2025Article
- CRISPR/Cas9 Protocols for Disrupting Gene Function in the Non-vertebrate Chordate Ciona.Integrative and comparative biology · 2024Review
- Harnessing the potential of mutation breeding, CRISPR genome editing, and beyond for sustainable agriculture.Functional & integrative genomics · 2024Review
- Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects.Microorganisms · 2024Review
- Establishment of a developmental atlas and transgenetic tools in the ascidianMarine life science & technology · 2023Article
- Marine animal evolutionary developmental biology-Advances through technology development.Evolutionary applications · 2023Article
- CatSper mediates not only chemotactic behavior but also the motility of ascidian sperm.Frontiers in cell and developmental biology · 2023Article
- Pax3/7 regulates neural tube closure and patterning in a non-vertebrate chordate.Frontiers in cell and developmental biology · 2022Article
- Advances in Nanoparticle Drug Delivery Systems for Anti-Hepatitis B Virus Therapy: A Narrative Review.International journal of molecular sciences · 2021Review
- Gene insertion in3 Biotech · 2021Article
- Quantitative Dissection of the ProximalFrontiers in cell and developmental biology · 2021Article
- Fertilization of Ascidians: Gamete Interaction, Self/Nonself Recognition and Sperm Penetration of Egg Coat.Frontiers in cell and developmental biology · 2021Review
- Phylogenetic Analyses of Glycosyl Hydrolase Family 6 Genes in Tunicates: Possible Horizontal Transfer.Genes · 2020Article
- Delivery methods for CRISPR/Cas9 gene editing in crustaceans.Marine life science & technology · 2020Article
- The notochord gene regulatory network in chordate evolution: Conservation and divergence from Ciona to vertebrates.Current topics in developmental biology · 2020Review
- Single-cell transcriptome profiling of the Ciona larval brain.Developmental biology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knockout of genes with CRISPR/Cas9 is a newly emerged approach to investigate functions of genes in various organisms. We demonstrate that CRISPR/Cas9 can mutate endogenous genes of the ascidian Ciona intestinalis, a splendid model for elucidating molecular mechanisms for constructing the chordate body plan. Short guide RNA (sgRNA) and Cas9 mRNA, when they are expressed in Ciona embryos by means of microinjection or electroporation of their expression vectors, introduced mutations in the target genes. The specificity of target choice by sgRNA is relatively high compared to the reports from some other organisms, and a single nucleotide mutation at the sgRNA dramatically reduced mutation efficiency at the on-target site. CRISPR/Cas9-mediated mutagenesis will be a powerful method to study gene functions in Ciona along with another genome editing approach using TALE nucleases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.