ArticleBiochemistry2014
SR-BI/CD36 chimeric receptors define extracellular subdomains of SR-BI critical for cholesterol transport.
Article in Biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Is reverse cholesterol transport regulated by active cholesterol?Journal of lipid research · 2023Review
- SR-B1's Next Top Model: Structural Perspectives on the Functions of the HDL Receptor.Current atherosclerosis reports · 2022Review
- Proline residues in scavenger receptor-BI's C-terminal region support efficient cholesterol transport.The Biochemical journal · 2019Article
- Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.Journal of lipid research · 2018Review
- Radiolabeled cholesteryl ethers: A need to analyze for biological stability before use.Biochemistry and biophysics reports · 2018Article
- Intracellular and Plasma Membrane Events in Cholesterol Transport and Homeostasis.Journal of lipids · 2018Review
- Lysosomal integral membrane protein-2 as a phospholipid receptor revealed by biophysical and cellular studies.Nature communications · 2017Article
- SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis.Trends in endocrinology and metabolism: TEM · 2017Review
- High-density lipoprotein receptor SCARB1 is required for carotenoid coloration in birds.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- NMR Structure of the C-Terminal Transmembrane Domain of the HDL Receptor, SR-BI, and a Functionally Relevant Leucine Zipper Motif.Structure (London, England : 1993) · 2017Article
- Shunts, channels and lipoprotein endosomal traffic: a new model of cholesterol homeostasis in the hepatocyte.Journal of biomedical research · 2017Article
- SCARB1 Gene Variants Are Associated With the Phenotype of Combined High High-Density Lipoprotein Cholesterol and High Lipoprotein (a).Circulation. Cardiovascular genetics · 2016Article
- Tryptophan 415 Is Critical for the Cholesterol Transport Functions of Scavenger Receptor BI.Biochemistry · 2016Article
- Scavenger receptor class B, type I-mediated uptake of A1AT by pulmonary endothelial cells.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Procollagen C-endopeptidase Enhancer Protein 2 (PCPE2) Reduces Atherosclerosis in Mice by Enhancing Scavenger Receptor Class B1 (SR-BI)-mediated High-density Lipoprotein (HDL)-Cholesteryl Ester Uptake.The Journal of biological chemistry · 2015Article
- Micellar lipid composition affects micelle interaction with class B scavenger receptor extracellular loops.Journal of lipid research · 2015Article
- Importance of Apolipoprotein A-I in Multiple Sclerosis.Frontiers in pharmacology · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
High-density lipoproteins (HDLs) are athero-protective, primarily because of their ability to promote cholesterol flux from peripheral tissues to the liver by reverse cholesterol transport (RCT). The delivery of HDL-cholesteryl esters (CE) into cells is mediated by the HDL receptor, scavenger receptor class B type I (SR-BI), a promising target for enhancing whole body cholesterol disposal and preventing cardiovascular disease. A detailed understanding of the structural determinants underlying proper SR-BI/HDL alignment that supports the selective uptake of HDL-CE into cells remains lacking. To this end, we exploited CD36, a class B scavenger receptor with a predicted topology similar to that of SR-BI that binds HDL but is unable to mediate efficient selective uptake of HDL-CE. We generated a series of SR-BI/CD36 chimeric receptors that span the extracellular (EC) domain of SR-BI to delineate regions that are essential for SR-BI's cholesterol transport functions. All 16 SR-BI/CD36 chimeras were transiently expressed in COS-7 cells, and their plasma membrane localization was confirmed. The majority of SR-BI/CD36 chimeric receptors displayed significant reductions in their ability to (i) bind HDL, (ii) deliver HDL-CE to cells, (iii) mediate efflux of free cholesterol (FC) to HDL, and (iv) redistribute plasma membrane domains of FC. We also demonstrated that changes in SR-BI function were independent of receptor oligomerization. Altogether, we have identified discrete subdomains, particularly in the N-terminal and C-terminal regions of the EC domain of SR-BI, that are critical for productive receptor-ligand interactions and the various cholesterol transport functions of SR-BI.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.